| WB | 1/500-1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | KBF1; EBP-1; NF-kB; CVID12; NF-kB1; NFKB-p50; NFkappaB; NF-kappaB; NFKB-p105; NF-kappa-B1; NF-kappabeta |
| WB Predicted band size | 105 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Fusion protein of human NFKB1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇关于NFKB1抗体的代表性文献摘要(模拟示例，非真实文献)：
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1. **文献名称**：*NF-κB1/p50 regulates macrophage inflammation in sepsis*
**作者**：Smith A, et al.
**摘要**：研究利用NFKB1特异性抗体进行Western blot和免疫荧光染色，发现NFKB1/p50在脓毒症模型中通过调控巨噬细胞TLR4信号通路介导炎症反应，抗体验证了其核转位机制。
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2. **文献名称**：*A functional screen identifies kinases modulating NF-κB1 activity*
**作者**：Chen L, et al.
**摘要**：通过CRISPR筛选结合NFKB1抗体染色质免疫共沉淀(ChIP)，发现IKKε磷酸化NFKB1并增强其与DNA结合能力，抗体特异性验证为实验关键步骤。
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3. **文献名称**：*NFKB1 antibody-based diagnostic panel for autoimmune diseases*
**作者**：Wang X, et al.
**摘要**：开发了一种高灵敏度NFKB1单克隆抗体，用于ELISA检测类风湿性关节炎患者血清中NFKB1的异常表达，证明其作为疾病生物标志物的潜力。
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注：以上为模拟摘要，实际文献需通过PubMed或Google Scholar以“NFKB1 antibody”为关键词检索。真实文献推荐参考《Nature Immunology》《Cell Reports》等期刊近年论文。
Nuclear factor kappa B subunit 1 (NFKB1) is a critical component of the NF-κB transcription factor family, which regulates immune response, inflammation, cell proliferation, and apoptosis. The NFKB1 gene encodes a precursor protein, p105. that is processed into the mature p50 subunit. This subunit forms heterodimers (commonly with RelA/p65) or homodimers to bind DNA and modulate gene expression. The canonical NF-κB pathway, involving p50. is activated by stimuli like cytokines (e.g., TNF-α, IL-1), pathogens, or stress signals, leading to the release of NF-κB from its inhibitor (IκB) and translocation to the nucleus.
NFKB1-specific antibodies are essential tools for studying this pathway. They are widely used in techniques such as Western blotting, immunohistochemistry, immunofluorescence, and chromatin immunoprecipitation (ChIP) to detect p105/p50 expression, localization, and DNA-binding activity. These antibodies help elucidate NFKB1's role in diseases, including cancer, autoimmune disorders, and chronic inflammation. For instance, dysregulated NFKB1 activity is linked to tumor progression, resistance to apoptosis, and excessive inflammatory responses.
Researchers must validate NFKB1 antibodies for specificity, as cross-reactivity with other NF-κB subunits (e.g., p52 from NFKB2) can occur. Applications often require context-dependent optimization, such as using nuclear/cytoplasmic fractionation to assess activation status. Knockout controls or siRNA-mediated silencing are recommended to confirm antibody reliability. Understanding NFKB1 dynamics through these tools advances therapeutic targeting of NF-κB pathways in disease treatment.
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