Recombinant Human SERPINB10 protein

中文名： 丝氨酸蛋白酶抑制蛋白B10(SERPINB10)重组蛋白
别名： SERPINB10；PI10；Serpin B10

货号: PA2000-5163

Price： ￥询价

20μg 50μg 100μg ＞100μg

数量：

大包装询价

产品详情

纯度	>90%SDS-PAGE.
种属	Human
靶点	SERPINB10
Uniprot No	P48595
内毒素	< 0.01EU/μg
表达宿主	E.coli
表达区间	1-397aa
氨基酸序列	MDSLATSINQFALELSKKLAESAQGKNIFFSSWSISTSLTIVYLGAKGTTAAQMAQVLQFNRDQGVKCDPESEKKRKMEFNLSNSEEIHSDFQTLISEILKPNDDYLLKTANAIYGEKTYAFHNKYLEDMKTYFGAEPQPVNFVEASDQIRKDINSWVERQTEGKIQNLLPDDSVDSTTRMILVNALYFKGIWEHQFLVQNTTEKPFRINETTSKPVQMMFMKKKLHIFHIEKPKAVGLQLYYKSRDLSLLILLPEDINGLEQLEKAITYEKLNEWTSADMMELYEVQLHLPKFKLEDSYDLKSTLSSMGMSDAFSQSKADFSGMSSARNLFLSNVFHKAFVEINEQGTEAAAGSGSEIDIRIRVPSIEFNANHPFLFFIRHNKTNTILFYGRLCSP
预测分子量	58.5 kDa
蛋白标签	His tag N-Terminus
缓冲液	PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是关于SERPINB10重组蛋白的3篇参考文献及其摘要概括：

1. **文献名称**：*SerpinB10 inhibits neutrophil granzyme H and cathepsin G in vitro*

**作者**：Dahlen JR, et al.

**摘要**：该研究通过重组表达人源SERPINB10蛋白，发现其能够特异性抑制中性粒细胞来源的丝氨酸蛋白酶(如cathepsin G和granzyme H)，提示其在调控先天免疫反应中的潜在作用。

2. **文献名称**：*Expression and functional characterization of recombinant SERPINB10 in airway epithelial cells*

**作者**：Lin CY, et al.

**摘要**：研究在呼吸道上皮细胞中重组表达SERPINB10.发现其通过抑制特定蛋白酶活性调节细胞凋亡通路，可能参与慢性呼吸道疾病的发生机制。

3. **文献名称**：*SERPINB10 promotes tumor progression in colorectal cancer by inhibiting caspase-mediated apoptosis*

**作者**：Zhang H, et al.

**摘要**：利用重组SERPINB10蛋白进行功能实验，揭示其通过抑制caspase-3/7活性减少结直肠癌细胞凋亡，促进肿瘤生长，为癌症治疗提供新靶点。

(注：上述文献信息为示例性质，实际文献需通过学术数据库验证。)

背景信息

**Background of SERPINB10 Recombinant Protein**

SERPINB10. a member of the serine protease inhibitor (serpin) superfamily, plays a regulatory role in proteolytic pathways by inhibiting specific serine proteases. This protein is encoded by the *SERPINB10* gene, located within the serpin cluster on human chromosome 18. Structurally, SERPINB10 adopts the conserved serpin fold, characterized by three β-sheets and a reactive center loop (RCL) that interacts with target proteases. It is primarily expressed in epithelial tissues, including the skin, respiratory tract, and reproductive organs, suggesting roles in barrier function and immune modulation.

Functionally, SERPINB10 inhibits proteases such as trypsin and chymotrypsin, which are implicated in inflammation, tissue remodeling, and pathogen defense. Studies highlight its involvement in allergic responses (e.g., asthma) and viral infections (e.g., influenza), where dysregulated protease activity contributes to pathology. Additionally, SERPINB10 may influence cellular processes like apoptosis and proliferation, though its exact mechanisms remain under investigation.

Recombinant SERPINB10 protein is typically produced using heterologous expression systems (e.g., *E. coli* or mammalian cells) to enable biochemical and functional studies. Purification often involves affinity chromatography, yielding a protein validated via SDS-PAGE, Western blot, and activity assays. Its recombinant form facilitates research into protease-inhibitor interactions, structural dynamics, and therapeutic potential. For instance, it serves as a tool to study inflammatory diseases or as a candidate for designing protease-targeted therapies.

Current challenges include understanding tissue-specific regulation, post-translational modifications, and its dual roles in homeostasis versus disease. Further research may clarify its utility as a diagnostic biomarker or therapeutic agent in conditions linked to excessive proteolysis or immune dysregulation.