| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | PIG27 |
| Entrez GeneID | 10126 |
| clone | 3C10A4 |
| WB Predicted band size | 12kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human DNAL4 (AA: 1-105) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是3篇关于DNAL4抗体的模拟参考文献(非真实文献，仅供示例参考)：
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1. **文献名称**：*DNAL4 as a critical component of cytoplasmic dynein in ciliary transport*
**作者**：Smith A, et al.
**摘要**：本研究利用特异性DNAL4抗体，通过免疫荧光和共聚焦显微镜技术，证实了DNAL4在纤毛内运输中的作用，并发现其缺失导致纤毛结构异常和信号传导功能障碍。
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2. **文献名称**：*Development and validation of a monoclonal antibody against human DNAL4 for cancer biomarker studies*
**作者**：Chen L, et al.
**摘要**：文章报道了一种高亲和力抗人DNAL4单克隆抗体的开发，验证了其在免疫组化(IHC)和Western blot中的应用，并发现DNAL4在乳腺癌组织中的表达水平与转移潜能相关。
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3. **文献名称**：*DNAL4 dysfunction disrupts spermatogenesis: Insights from antibody-mediated localization analysis*
**作者**：Tanaka K, et al.
**摘要**：通过DNAL4抗体定位分析，揭示了DNAL4在小鼠精子形成过程中参与鞭毛动力蛋白组装的机制，其表达异常可能导致雄性不育。
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(注：以上文献信息为模拟生成，实际研究中请通过PubMed、Google Scholar等平台检索真实文献。)
The DNAL4 antibody targets dynein light chain 4 (DNAL4), a component of the cytoplasmic dynein complex, a motor protein critical for intracellular transport and mitotic processes. Cytoplasmic dyneins are multi-subunit complexes composed of heavy, intermediate, light intermediate, and light chains. DNAL4. a light chain subunit, facilitates cargo binding and regulates dynein-mediated retrograde transport along microtubules. It plays roles in organelle positioning, vesicle trafficking, and mitotic spindle organization. Dysregulation of dynein function is linked to neurodegenerative diseases, ciliopathies, and developmental disorders.
DNAL4 antibodies are widely used in research to study dynein complex assembly, localization, and interactions. They help investigate DNAL4's involvement in cellular processes like Golgi apparatus maintenance, neuronal transport, and cell division. Commercially available antibodies are validated for applications such as Western blotting, immunofluorescence, and immunoprecipitation. Studies using DNAL4 antibodies have also explored its potential role in primary ciliary dyskinesia and other ciliopathies, where dynein defects impair ciliary motility or structure.
As a molecular tool, DNAL4 antibodies contribute to understanding dynein-related pathologies and validating disease models. However, specificity validation (e.g., knockout controls) is essential due to structural similarities among dynein subunits. Ongoing research aims to clarify DNAL4's tissue-specific functions and its interplay with other dynein light chains.
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