| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 1/300 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | L31MT; L42MT; S32MT; MRPL31; MRPS32; PTD007; RPML31; HSPC204; MRP-L31; MRP-L42; MRP-S32 |
| Entrez GeneID | 28977 |
| clone | 3H6G11 |
| WB Predicted band size | 16.7kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human MRPL42 (AA: 142-203) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于MRPL42抗体的3篇参考文献示例(注：内容为模拟概括，实际文献需根据具体研究检索)：
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1. **文献名称**: *MRPL42 as a novel biomarker in colorectal cancer: Expression and functional analysis*
**作者**: Zhang Y, et al.
**摘要**: 本研究通过免疫组化(IHC)和Western blot技术，利用MRPL42特异性抗体检测其在结直肠癌组织中的表达升高，并发现其过表达与患者预后不良相关。机制研究表明，MRPL42通过调控线粒体功能促进肿瘤细胞增殖。
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2. **文献名称**: *Mitochondrial ribosomal protein L42 regulates apoptosis via interaction with Bcl-2 family proteins*
**作者**: Lee S, Kim JH.
**摘要**: 该研究使用MRPL42抗体进行免疫共沉淀(Co-IP)和免疫荧光实验，揭示了MRPL42与Bcl-2家族蛋白的相互作用，证明其通过线粒体途径调控细胞凋亡，为癌症治疗提供潜在靶点。
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3. **文献名称**: *Proteomic analysis of MRPL42 deficiency in HeLa cells using CRISPR/Cas9 and antibody-based validation*
**作者**: Wang X, et al.
**摘要**: 通过CRISPR/Cas9敲除MRPL42基因，结合Western blot(使用抗MRPL42抗体)和质谱分析，发现MRPL42缺失导致线粒体核糖体组装异常，并影响氧化磷酸化相关蛋白表达。
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如需获取真实文献，建议在PubMed或Google Scholar检索关键词“MRPL42 antibody”、“MRPL42 mitochondrial ribosome”或结合具体研究领域(如癌症、代谢疾病)。部分商业抗体公司(如Abcam、CST)的产品页面也可能提供相关引用文献。
The MRPL42 antibody is a research tool designed to detect mitochondrial ribosomal protein L42 (MRPL42), a key component of the mitochondrial ribosome’s large subunit. MRPL42 is encoded by nuclear DNA and plays a critical role in mitochondrial protein synthesis by facilitating the assembly and stability of the ribosome. Mitochondrial ribosomes are responsible for translating mitochondrial DNA-encoded genes, particularly those involved in oxidative phosphorylation (OXPHOS), which generates cellular energy (ATP). Dysregulation of MRPL42 or mitochondrial ribosomal proteins has been linked to mitochondrial dysfunction, metabolic disorders, and diseases such as cancer or neurodegenerative conditions.
The MRPL42 antibody is commonly used in techniques like Western blotting, immunofluorescence, or immunohistochemistry to study protein expression, localization, and interactions in various tissues or cell lines. Researchers utilize it to explore mitochondrial biology, metabolic pathways, or disease mechanisms. Antibodies targeting MRPL42 are typically produced in hosts like rabbits or mice, with validation steps (e.g., knockout controls) to ensure specificity. Commercial suppliers often provide datasheets detailing applications, species reactivity, and experimental conditions. Recent studies have employed MRPL42 antibodies to investigate its role in cellular stress responses, cancer progression, or rare mitochondrial disorders, highlighting its relevance in both basic and translational research.
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