| WB | 1/500 - 1/2000 | Human,Mouse,Rat |
| IF | 1/200-1/300 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 1/50 - 1/200 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | PDHA; PDHAD; PHE1A; PDHCE1A |
| Entrez GeneID | 5160 |
| clone | 1G10C10 |
| WB Predicted band size | 43 kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Purified recombinant fragment of human PDHA1(AA: 241-390) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于PDHA1抗体的示例参考文献(请注意，以下内容为模拟示例，非真实文献)：
1. **文献名称**：*"PDHA1-mediated glycolysis regulates mitochondrial metabolism in cancer cells"*
**作者**：Smith A, et al.
**摘要**：研究利用PDHA1特异性抗体，通过Western blot和免疫组化分析，揭示了PDHA1在癌细胞线粒体代谢中的关键作用，其表达下调与糖酵解活性和肿瘤进展相关。
2. **文献名称**：*"Phosphorylation-dependent regulation of PDHA1 activity in diabetic cardiomyopathy"*
**作者**：Chen L, et al.
**摘要**：通过PDHA1抗体检测磷酸化修饰位点，发现糖尿病心肌病中PDHA1活性受抑制的机制，为代谢异常相关心脏疾病提供新靶点。
3. **文献名称**：*"Structural insights into PDHA1 and antibody interactions for targeted therapy"*
**作者**：Johnson R, et al.
**摘要**：利用PDHA1抗体进行共结晶实验，解析了PDHA1蛋白表位结构，为开发针对PDHA1功能障碍的精准治疗策略奠定基础。
4. **文献名称**：*"PDHA1 as a biomarker in hepatocellular carcinoma: Validation by immunohistochemistry"*
**作者**：Wang Y, et al.
**摘要**：研究采用商业化PDHA1抗体对肝癌组织进行染色，证实PDHA1低表达与患者预后不良显著相关，提示其作为潜在诊断标志物的价值。
**备注**：以上为示例，实际文献需通过PubMed或Web of Science等平台检索关键词“PDHA1 antibody”或“PDHA1 function”获取。推荐验证真实文献的可用性及抗体货号(如CST #3205)。
The pyruvate dehydrogenase complex (PDC) is a critical mitochondrial enzyme complex that links glycolysis to the citric acid cycle by converting pyruvate to acetyl-CoA. PDHA1 (Pyruvate Dehydrogenase E1 Alpha 1 Subunit) encodes the α-subunit of the PDC's E1 component, which catalyzes the decarboxylation of pyruvate. Dysregulation of PDHA1 is linked to metabolic disorders, neurodegenerative diseases, and cancer, as altered pyruvate metabolism affects cellular energy balance and biosynthetic pathways.
PDHA1 antibodies are essential tools for studying the expression, localization, and post-translational modification (e.g., phosphorylation) of this subunit. These antibodies are widely used in Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to assess PDHA1 levels in tissues or cell lines, particularly in research on metabolic reprogramming in cancers (e.g., the Warburg effect) or inherited PDHA1 deficiencies causing lactic acidosis and neurological impairment.
Many PDHA1 antibodies are validated for specificity across human, mouse, and rat samples, with some targeting phosphorylated residues (e.g., Ser293) to study regulatory mechanisms. Researchers rely on these antibodies to explore therapeutic strategies targeting metabolic pathways or to diagnose genetic mutations affecting PDC activity. Proper controls, such as siRNA knockdown or PDHA1-deficient samples, are recommended to confirm antibody specificity in experimental setups.
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