| WB | 1/500 - 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 1/50 - 1/200 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | PDHA; PDHAD; PHE1A; PDHCE1A |
| Entrez GeneID | 5160 |
| clone | 4E12D6 |
| WB Predicted band size | 43 kDa |
| Host/Isotype | Mouse IgG2a |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Purified recombinant fragment of human PDHA1(AA: 241-390) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于PDHA1抗体的模拟参考文献示例(仅供参考，非真实文献)：
1. **"PDHA1 expression modulates metabolic plasticity in triple-negative breast cancer"**
- **作者**: Smith A, et al.
- **摘要**: 研究通过Western blot和免疫组化(使用PDHA1抗体)发现，PDHA1在乳腺癌细胞中表达降低与糖酵解增强相关，提示其表达缺失可能驱动Warburg效应。
2. **"Phosphorylation-dependent regulation of PDHA1 activity in mitochondrial metabolism"**
- **作者**: Chen L, et al.
- **摘要**: 利用特异性PDHA1抗体分析磷酸化位点，揭示PDHA1活性通过激酶调控影响细胞氧化代谢，为代谢疾病治疗提供新靶点。
3. **"PDHA1 deficiency screening using antibody-based assays in pediatric patients"**
- **作者**: Gonzalez R, et al.
- **摘要**: 开发基于PDHA1抗体的ELISA检测方法，用于快速诊断遗传性PDHA1缺陷导致的乳酸酸中毒，验证其临床实用性。
4. **"Targeting PDHA1 sensitizes glioblastoma cells to oxidative stress"**
- **作者**: Kim H, et al.
- **摘要**: 通过shRNA和PDHA1抗体验证，抑制PDHA1可增强胶质母细胞瘤对氧化应激的敏感性，提示其作为联合治疗靶标的潜力。
如需真实文献，建议在PubMed或Google Scholar中检索关键词“PDHA1 antibody”或“PDHA1 metabolism”，筛选应用该抗体的实验性研究。
The PDHA1 antibody is a crucial tool in studying the pyruvate dehydrogenase complex (PDC), a key enzyme system central to cellular energy metabolism. PDHA1 encodes the E1α subunit of PDC, which catalyzes the conversion of pyruvate to acetyl-CoA in the mitochondrial matrix, linking glycolysis to the tricarboxylic acid (TCA) cycle. This reaction is critical for ATP production and biosynthetic pathways. Dysregulation of PDHA1 is implicated in metabolic disorders, cancers, and neurodegenerative diseases.
PDHA1 antibodies are widely used in research to detect protein expression, localization, and post-translational modifications (e.g., phosphorylation at Ser293. which inhibits PDC activity). They enable techniques like Western blotting, immunohistochemistry, and immunofluorescence, aiding investigations into metabolic reprogramming in cancer (e.g., the Warburg effect) or mitochondrial dysfunction. Commercially available antibodies are typically raised against human PDHA1 epitopes, validated for specificity in model organisms.
Mutations in PDHA1 cause pyruvate dehydrogenase deficiency, an X-linked disorder characterized by lactic acidosis and neurological impairment. Antibodies help diagnose such conditions by assessing protein levels in patient samples. Additionally, PDHA1 is explored as a therapeutic target; its antibodies assist in drug development studies. Researchers must validate these antibodies rigorously due to potential cross-reactivity with homologous proteins (e.g., PDHA2) or isoforms, ensuring accurate interpretation of metabolic studies.
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