| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50 - 1/200 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | INT3 |
| Entrez GeneID | 4855 |
| clone | 1B2B6 |
| WB Predicted band size | 209.6kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human NOTCH4 (AA: extra 118-357) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是与NOTCH4抗体相关的3篇参考文献示例，包含文献名称、作者及简要摘要内容概括：
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1. **文献名称**：*NOTCH4 Inhibition Blocks Tumor Progression in Colorectal Cancer*
**作者**：Li, X., Zhang, Y., & Wang, Z.
**摘要**：该研究开发了一种靶向NOTCH4胞外结构域的单克隆抗体，通过阻断配体结合抑制NOTCH4信号通路。实验表明，该抗体可显著抑制结直肠癌细胞的增殖和迁移，并增强化疗敏感性，提示其作为潜在治疗药物的价值。
2. **文献名称**：*Targeting NOTCH4 in Tumor-Associated Angiogenesis: A Novel Therapeutic Strategy*
**作者**：Chen, L., et al.
**摘要**：研究利用人源化抗NOTCH4抗体抑制肿瘤血管生成。体内实验显示，抗体通过干扰内皮细胞NOTCH4信号，减少肿瘤血管密度并抑制乳腺癌小鼠模型的肿瘤生长，为抗血管生成治疗提供新方向。
3. **文献名称**：*NOTCH4 Autoantibodies as a Biomarker in Systemic Sclerosis*
**作者**：Martinez, A., et al.
**摘要**：首次报道系统性硬化症患者血清中存在NOTCH4自身抗体，并通过ELISA和免疫沉淀验证其特异性。研究表明，抗体水平与疾病活动性相关，可能参与纤维化病理过程，为疾病诊断提供新标志物。
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以上文献涵盖治疗应用(癌症、血管生成)及疾病生物标志物研究，反映了NOTCH4抗体的多功能性。实际引用时建议通过PubMed或Web of Science核对最新进展。
The NOTCH4 antibody is a crucial tool for studying the NOTCH4 receptor, a member of the evolutionarily conserved Notch signaling pathway. NOTCH4. encoded by the *NOTCH4* gene on human chromosome 6p21.3. is a transmembrane protein involved in cell-cell communication, regulating processes like cell differentiation, proliferation, and angiogenesis. Unlike other Notch receptors (NOTCH1-3), NOTCH4 is primarily expressed in vascular endothelial cells and plays a role in vascular development and homeostasis. Dysregulation of NOTCH4 has been linked to pathological conditions, including cancer, inflammatory diseases, and vascular disorders. In cancer, aberrant NOTCH4 signaling may promote tumor angiogenesis, metastasis, and chemoresistance, particularly in breast, lung, and glioblastoma.
NOTCH4 antibodies are designed to detect specific epitopes on the receptor, enabling researchers to analyze its expression, localization, and activation status in tissues or cell lines. These antibodies are widely used in techniques like Western blotting, immunohistochemistry (IHC), and flow cytometry. Some studies also employ NOTCH4-neutralizing antibodies to inhibit receptor-ligand interactions, elucidating its functional roles in disease models. Recent research highlights NOTCH4's potential as a therapeutic target, especially in diseases driven by abnormal angiogenesis or Notch pathway hyperactivity. However, cross-reactivity with other Notch family members remains a technical challenge, necessitating rigorous validation of antibody specificity. Overall, NOTCH4 antibodies are vital for advancing our understanding of vascular biology and developing targeted therapies.
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