| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 1/200 - 1/1000 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | IIAE7 |
| Entrez GeneID | 3661 |
| clone | 2A10A8 |
| WB Predicted band size | 47.2kDa |
| Host/Isotype | Mouse IgG2b |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Purified recombinant fragment of human IRF3 (AA: 1-150) expressed in HEK293-6e cells supernatant. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于IRF3抗体的3篇参考文献及其摘要概括：
1. **文献名称**：*"IRF3 activation via phosphorylation by the antiviral kinase TBK1"*
**作者**：Sato, M. et al.
**摘要**：研究阐明了IRF3在抗病毒免疫中的激活机制，通过TBK1介导的磷酸化促进其核转位。实验中利用IRF3抗体检测其磷酸化状态及亚细胞定位。
2. **文献名称**：*"Viral recognition by TLRs and cytoplasmic RNA sensors: role of IRF3 in type I IFN responses"*
**作者**：Honda, K. et al.
**摘要**：探讨病毒识别通路中IRF3的核心作用，通过Western blot和免疫荧光技术(使用IRF3抗体)揭示其在TLR和RLR信号中的激活动态。
3. **文献名称**：*"Regulation of IRF3 activation in antiviral innate immunity"*
**作者**：Servant, M.J. et al.
**摘要**：分析IRF3的翻译后修饰(如泛素化)对抗病毒反应的影响，通过IRF3抗体进行免疫共沉淀实验，揭示其与下游信号分子的相互作用。
4. **文献名称**：*"IRF3-dependent cross-talk between innate antiviral and DNA damage responses"*
**作者**：Zeng, W. et al.
**摘要**：研究IRF3在DNA损伤与抗病毒信号中的双重角色，使用IRF3抗体验证其在DNA损伤条件下的异常激活及功能关联。
这些文献均通过IRF3抗体探究其在先天免疫中的分子机制，涵盖磷酸化检测、亚细胞定位及蛋白互作分析等应用场景。
Interferon Regulatory Factor 3 (IRF3) is a key transcription factor in innate immune responses, particularly in antiviral signaling. It is normally sequestered in the cytoplasm in an inactive form. Upon viral infection or detection of pathogen-associated molecular patterns (PAMPs), pattern recognition receptors (e.g., TLR3. RIG-I) trigger signaling cascades that lead to IRF3 phosphorylation. This post-translational modification enables IRF3 dimerization, nuclear translocation, and activation of type I interferons (IFNs) and pro-inflammatory cytokines, critical for host defense.
IRF3 antibodies are essential tools for studying its expression, activation status, and subcellular localization. They are widely used in techniques like Western blotting, immunofluorescence, and immunoprecipitation to investigate IRF3's role in immune signaling pathways. Phospho-specific IRF3 antibodies (e.g., recognizing Ser386 phosphorylation) are particularly valuable for distinguishing activated IRF3 from its inactive form. Researchers also employ these antibodies to explore IRF3 dysregulation in diseases, including viral evasion mechanisms, autoimmune disorders, and cancer immunotherapy studies. However, users must validate antibody specificity due to potential cross-reactivity with homologous proteins (e.g., IRF7) and ensure proper controls (e.g., IRF3-knockout cells) in experiments.
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