| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | CL2; DCK2; CLIK2; DCDC3; CLICK2; DCDC3B; DCAMKL2; CLICK-II |
| Entrez GeneID | 166614 |
| clone | 3H1G4 |
| WB Predicted band size | 83.6kDa |
| Host/Isotype | Mouse IgG2a |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human DCLK2 (AA: 652-766) expressed in mammalian. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
+ +
以下是关于DCLK2抗体的3篇参考文献示例(内容基于公开研究归纳，非真实文献)：
1. **文献名称**：*DCLK2 as a Marker for Colorectal Cancer Stem Cells*
**作者**：Smith A, et al.
**摘要**：研究利用DCLK2特异性抗体识别结直肠癌干细胞群体，通过免疫组化证实DCLK2高表达与肿瘤转移和耐药性相关，提示其作为治疗靶点的潜力。
2. **文献名称**：*DCLK2 Isoforms in Neuronal Development: Antibody Validation and Functional Analysis*
**作者**：Chen L, et al.
**摘要**：开发并验证了两种DCLK2亚型特异性抗体，通过Western blot和免疫荧光证明DCLK2在小鼠大脑海马区神经发生中的动态表达，揭示其调控神经元迁移的作用。
3. **文献名称**：*Comparative Study of DCLK1 and DCLK2 Antibodies in Pancreatic Cancer Models*
**作者**：Wang Y, et al.
**摘要**：对比DCLK1与DCLK2抗体在胰腺癌组织中的特异性，发现DCLK2抗体可选择性标记侵袭性亚群，并通过基因敲除实验验证其功能与肿瘤微环境调控相关。
(注：以上为模拟示例，实际文献需通过PubMed/Google Scholar等平台检索确认。)
Doublecortin-like kinase 2 (DCLK2) is a microtubule-associated protein belonging to the doublecortin (DCX) family, characterized by tandem doublecortin domains and a serine/threonine kinase domain. It shares structural homology with DCLK1 but differs in expression patterns and functional roles. DCLK2 is implicated in neuronal development, regulating microtubule dynamics critical for axon guidance, dendritic branching, and neuronal migration. Recent studies highlight its involvement in cancer progression, stem cell maintenance, and neurodegenerative disorders.
DCLK2 antibodies are essential tools for detecting DCLK2 expression and localization in tissues or cell lines. They enable research on its dual role as a structural regulator and signaling kinase, particularly in pathways like MAPK/ERK and Wnt/β-catenin. In cancer, DCLK2 overexpression correlates with tumor aggressiveness in pancreatic, colorectal, and ovarian cancers, making it a potential therapeutic target or biomarker. Antibodies against specific isoforms (e.g., full-length vs. truncated) help dissect their distinct functions. Challenges in antibody development include cross-reactivity with DCLK1 and distinguishing post-translational modifications. Validated DCLK2 antibodies support diagnostic assays, mechanistic studies, and preclinical drug evaluation, advancing understanding of its pathophysiological roles.
×
