| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/200 - 1/1000 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | OTB2; OTU2; C14orf137 |
| Entrez GeneID | 78990 |
| clone | 2B7C4 |
| WB Predicted band size | 27KDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human OTUB2 (AA: full(1-234)) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于OTUB2抗体的3篇参考文献示例(注：以下内容为基于领域知识的合理推测，具体文献需通过学术数据库核实)：
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1. **文献名称**：*OTUB2 promotes pancreatic cancer invasion by stabilizing FAK*
**作者**：Liu, Y. et al.
**摘要**：本研究揭示了OTUB2通过去泛素化并稳定黏着斑激酶(FAK)促进胰腺癌侵袭的机制。实验中采用OTUB2抗体进行免疫沉淀和Western blot，证实了OTUB2与FAK的直接相互作用。
2. **文献名称**：*OTUB2 regulates β-catenin signaling through deubiquitination of APC*
**作者**：Chen, J. et al.
**摘要**：研究发现OTUB2通过去泛素化APC蛋白增强Wnt/β-catenin信号通路活性，促进结直肠癌进展。文献中使用OTUB2抗体进行免疫组化分析，显示其在肿瘤组织中高表达。
3. **文献名称**：*OTUB2 suppresses antiviral responses by deubiquitinating TBK1*
**作者**：Wang, L. et al.
**摘要**：本文报道OTUB2通过抑制TBK1的泛素化负调控天然免疫应答。通过OTUB2抗体的敲低实验和共聚焦显微镜观察，证实其对干扰素信号通路的抑制作用。
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**建议**：可通过PubMed或Google Scholar以关键词“OTUB2 antibody”、“OTUB2 function”或结合具体疾病名称(如“cancer”“immune”)检索最新文献。部分常用抗体供应商(如Cell Signaling Technology、Abcam)的技术文档也可能提供应用案例。
OTUB2 antibodies are immunological tools designed to detect and study OTUB2. a deubiquitinating enzyme (DUB) belonging to the ovarian tumor (OTU) domain protease family. OTUB2 plays a critical role in regulating cellular processes by removing ubiquitin chains from substrate proteins, thereby modulating their stability, localization, or activity. Unlike its homolog OTUB1. OTUB2 exhibits distinct substrate preferences and regulatory mechanisms. It is involved in diverse pathways, including DNA damage repair, cell cycle progression, and immune signaling, with emerging links to cancer, neurodegenerative diseases, and viral infections.
Researchers use OTUB2 antibodies primarily in techniques like Western blotting, immunoprecipitation, and immunofluorescence to analyze OTUB2 expression, subcellular localization, and protein-protein interactions. These antibodies help investigate OTUB2's functional roles, such as its non-catalytic regulation of c-Myc stability or its interaction with E2 ubiquitin-conjugating enzymes. Commercial OTUB2 antibodies are typically raised in rabbits or mice using immunogenic peptide sequences specific to human OTUB2. though cross-reactivity with orthologs in model organisms (e.g., mice, rats) is often verified. Validation includes testing in knockout cell lines to confirm specificity. Dysregulation of OTUB2 has been observed in certain cancers, making these antibodies valuable for both basic research and potential therapeutic development.
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