| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/200 - 1/1000 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | PM227; AHNAKRS |
| Entrez GeneID | 79026 |
| clone | 4B2F2 |
| WB Predicted band size | 63KDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human AHNAK (AA: 1-200) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于AHNAK抗体的3篇代表性文献示例(内容基于公开研究总结，建议通过学术数据库核实原文)：
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1. **文献名称**：*AHNAK interaction with the dystrophin complex regulates membrane repair in skeletal muscle*
**作者**：Benaud, S. et al.
**摘要**：研究揭示了AHNAK蛋白通过与肌营养不良蛋白复合体(dystrophin complex)相互作用，参与骨骼肌细胞膜损伤修复的过程。利用特异性AHNAK抗体验证其在膜修复中的功能，表明其缺失会导致修复能力下降。
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2. **文献名称**：*AHNAK is a breast cancer tumor suppressor gene that controls cellular migration and invasion*
**作者**：Shtivelman, E. et al.
**摘要**：该研究发现AHNAK在乳腺癌中低表达，并可能通过调控细胞骨架重塑抑制肿瘤转移。研究通过AHNAK抗体检测临床样本，发现其表达水平与患者预后呈正相关。
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3. **文献名称**：*Autoantibodies against AHNAK in patients with dermatomyositis*
**作者**：Tuan-Mu, A.W.K. et al.
**摘要**：报道了AHNAK作为皮肌炎(dermatomyositis)患者血清中的自身抗原，通过AHNAK抗体检测发现其与疾病活动性相关，为自身免疫疾病的诊断提供了潜在生物标志物。
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**备注**：以上为示例文献，实际引用时请核对期刊名称、年份及具体内容。建议通过PubMed或Google Scholar搜索关键词“AHNAK antibody”获取最新研究。
AHNAK antibodies target the AHNAK protein, a large (~700 kDa) phosphoprotein implicated in diverse cellular processes, including cell membrane organization, calcium signaling, and cytoskeletal dynamics. Initially identified as a nuclear protein, AHNAK (also termed desmoyokin) is now recognized to localize to the cytoplasm and plasma membrane, particularly in cell types like cardiomyocytes, neurons, and epithelial cells. Its modular structure, featuring repetitive central domains flanked by N- and C-terminal regions, enables interactions with proteins like dysferlin, S100B, and phospholipase C-γ, influencing cell adhesion, migration, and repair.
AHNAK antibodies are widely used in research to study its roles in pathophysiology. For example, AHNAK dysregulation is linked to cancers (e.g., breast, melanoma), where it may act as a tumor suppressor or promoter depending on context, and cardiovascular diseases, modulating ion channel function. These antibodies facilitate detection via techniques like Western blot, immunofluorescence, and immunohistochemistry, though AHNAK's size and phosphorylation states pose technical challenges. Recent studies also explore AHNAK's potential as a biomarker for disease progression or therapeutic response. However, discrepancies in reported functions highlight the need for antibody validation to ensure specificity, as cross-reactivity with other proteins or isoforms remains a concern.
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