| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CAMK2D |
| Uniprot No | Q13557 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-499aa |
| 氨基酸序列 | ASTTTCTRFTDEYQLFEELGKGAFSVVRRCMKIPTGQEYAAKIINTKKLSARDHQKLEREARICRLLKHPNIVRLHDSISEEGFHYLVFDLVTGGELFEDIVAREYYSEADASHCIQQILESVNHCHLNGIVHRDLKPENLLLASKSKGAAVKLADFGLAIEVQGDQQAWFGFAGTPGYLSPEVLRKDPYGKPVDMWACGVILYILLVGYPPFWDEDQHRLYQQIKAGAYDFPSPEWDTVTPEAKDLINKMLTINPAKRITASEALKHPWICQRSTVASMMHRQETVDCLKKFNARRKLKGAILTTMLATRNFSAAKSLLKKPDGVKESTESSNTTIEDEDVKARKQEIIKVTEQLIEAINNGDFEAYTKICDPGLTAFEPEALGNLVEGMDFHRFYFENALSKSNKPIHTIILNPHVHLVGDDAACIAYIRLTQYMDGSGMPKTMQSEETRVWHRRDGKWQNVHFHRSGSPTVPIKPPCIPNGKENFSGGTSLWQNI |
| 预测分子量 | 63.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于CAMK2D重组蛋白的关键文献概览：
1. **文献名称**：*Structural insights into the autoinhibition and phosphorylation-induced activation of CaMKIIδ*
**作者**：Chao LH 等(2021)
**摘要**：通过重组CAMK2D蛋白的晶体结构解析，揭示了其自抑制机制及磷酸化后激活的关键构象变化，为心脏疾病相关突变研究提供结构基础。
2. **文献名称**：*CaMKIIδ drives cardiomyocyte hypertrophy through phosphorylation of HDAC4 and suppression of fetal gene expression*
**作者**：Backs J 等(2017)
**摘要**：利用重组CAMK2D蛋白进行体外激酶实验，证明其通过磷酸化组蛋白去乙酰化酶HDAC4.调控心肌细胞肥大的基因表达通路。
3. **文献名称**：*Recombinant CaMKIIδ protein purification and functional characterization in cancer cell migration*
**作者**：Li Y 等(2019)
**摘要**：报道了重组CAMK2D蛋白的优化表达纯化方法，并发现其通过激活RhoA信号通路促进乳腺癌细胞迁移，提示其作为癌症治疗靶点的潜力。
*注：以上文献标题和作者为虚构示例，实际研究需通过学术数据库(如PubMed)检索确认。CAMK2D重组蛋白研究多聚焦于结构解析、心血管/肿瘤疾病机制及药物筛选方向。*
**Background of CAMK2D Recombinant Protein**
CAMK2D (Calcium/Calmodulin-Dependent Protein Kinase II Delta) is a member of the CaMKII family, a group of serine/threonine kinases central to calcium-mediated signaling pathways. Expressed ubiquitously but predominantly in the heart, brain, and immune cells, CAMK2D plays critical roles in cellular processes such as excitation-contraction coupling, gene expression regulation, and synaptic plasticity. Its activation is triggered by calcium influx, which promotes binding to calmodulin, inducing autophosphorylation and sustained kinase activity even after calcium levels decline—a feature critical for long-term cellular responses.
The CAMK2D gene undergoes alternative splicing, yielding multiple isoforms (e.g., δB, δC) with distinct tissue-specific functions. Dysregulation of CAMK2D is implicated in cardiovascular diseases (e.g., arrhythmias, hypertrophy), neurodegenerative disorders, and cancer progression, making it a therapeutic target of interest.
Recombinant CAMK2D proteins are engineered using expression systems like *E. coli* or mammalian cells, ensuring post-translational modifications mimic native forms. These proteins retain enzymatic activity and structural integrity, enabling *in vitro* studies to dissect signaling mechanisms, screen inhibitors, or model disease pathways. Their applications span drug discovery, mechanistic studies of calcium signaling, and diagnostics.
Research on CAMK2D recombinant proteins has advanced understanding of its role in pathologies and highlighted its potential as a biomarker or therapeutic target. However, challenges remain in isoform-specific regulation and minimizing off-target effects in clinical applications. Continued exploration aims to refine its utility in precision medicine and disease intervention.
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