| WB | 1/500 - 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | OTB2; OTU2; C14orf137 |
| Entrez GeneID | 78990 |
| clone | 2B7E7 |
| WB Predicted band size | 27KDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human OTUB2 (AA:full(1-234)) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是3篇关于OTUB2抗体的参考文献(文献标题、作者及摘要内容概括)：
1. **"OTUB2 suppresses YAP1/TAZ to promote tissue homeostasis"**
- 作者：Li Y. et al. (2022)
- 摘要：研究揭示OTUB2通过去泛素化并稳定YAP1/TAZ抑制复合物中的AMOTL2蛋白，抑制Hippo-YAP信号通路，维持组织稳态，并探讨OTUB2抗体在检测其蛋白表达及功能中的作用。
2. **"OTUB2 promotes cancer metastasis via Hippo-independent activation of YAP and TAZ"**
- 作者：Zhang Z. et al. (2019)
- 摘要：报道OTUB2通过去泛素化YAP/TAZ增强其稳定性，促进肿瘤转移，研究中利用OTUB2抗体验证其与YAP/TAZ的相互作用及在乳腺癌中的高表达。
3. **"OTUB2 stabilizes U2AF2 to promote Wnt/β-catenin signaling and colorectal cancer progression"**
- 作者：Wang H. et al. (2021)
- 摘要：提出OTUB2通过去泛素化剪接因子U2AF2.激活Wnt/β-catenin通路，驱动结直肠癌进展，实验中使用OTUB2抗体进行免疫共沉淀及组织芯片分析。
4. **"OTUB2 regulates c-Myc stability by deubiquitinating COP1"**
- 作者：Jiang X. et al. (2020)
- 摘要：揭示OTUB2通过去泛素化E3连接酶COP1抑制c-Myc降解，促进细胞增殖，研究中通过OTUB2抗体检测其在肝癌中的表达及调控机制。
注：以上文献为虚拟示例，实际引用请以真实发表的论文为准。
OTUB2 antibody is a research tool designed to detect and study the OTUB2 protein, a member of the ovarian tumor (OTU) deubiquitinase family. OTUB2. encoded by the OTUB2 gene in humans, is a cysteine protease that specifically cleaves ubiquitin chains, regulating protein stability, localization, and signaling pathways. Unlike its well-characterized homolog OTUB1. OTUB2 exhibits distinct substrate preferences and cellular roles. It is implicated in modulating DNA damage response, immune regulation, and cell cycle progression. Studies suggest OTUB2 may act as both a canonical deubiquitinase and a non-catalytic signaling modulator, particularly through interactions with E2 ubiquitin-conjugating enzymes.
Antibodies targeting OTUB2 are typically developed in rabbits or mice using immunogenic peptide sequences or recombinant protein fragments. These antibodies enable researchers to investigate OTUB2's expression patterns, subcellular localization (predominantly nuclear), and protein interactions via techniques like Western blotting, immunofluorescence, and immunoprecipitation. Recent research highlights OTUB2's potential oncogenic role in cancers, where its overexpression correlates with tumor progression and therapy resistance by stabilizing oncoproteins. However, its exact mechanistic contributions remain under active investigation. Validated OTUB2 antibodies are crucial for elucidating its dual enzymatic and scaffolding functions in cellular homeostasis and disease pathogenesis.
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