Mouse Monoclonal PRF1 Antibody

The PRF1 antibody targets perforin-1. a cytotoxic effector protein encoded by the PRF1 gene. Perforin-1 is predominantly expressed in natural killer (NK) cells and cytotoxic T lymphocytes (CTLs), where it plays a critical role in immune defense by forming pores in target cell membranes. This pore-forming activity facilitates the entry of pro-apoptotic granzymes, triggering programmed cell death in virus-infected or malignant cells. PRF1 antibodies are essential tools for studying immune cell function, particularly in contexts like cancer immunology, viral infections, and autoimmune disorders. They are widely used in techniques such as Western blotting, immunohistochemistry (IHC), and flow cytometry to detect perforin-1 expression levels or localization in tissues and immune cells. Clinically, PRF1 mutations are linked to familial hemophagocytic lymphohistiocytosis (FHL type 2), a severe immunodeficiency disorder characterized by defective cytotoxic granule-mediated apoptosis. PRF1 antibodies aid in diagnosing such conditions by identifying protein deficiencies or abnormal expression patterns. Additionally, research applications include investigating immune evasion mechanisms in tumors and evaluating therapeutic interventions aimed at enhancing cytotoxic immune responses. Validation of PRF1 antibody specificity is crucial, as cross-reactivity with unrelated proteins may lead to inaccurate interpretations in both research and diagnostic settings.