| WB | 1/500 - 1/2000 | Human, Mouse, Rat, Monkey |
| IF | 咨询技术 | Human, Mouse, Rat, Monkey |
| IHC | 咨询技术 | Human, Mouse, Rat, Monkey |
| ICC | 1/200 - 1/1000 | Human, Mouse, Rat, Monkey |
| FCM | 1/200 - 1/400 | Human, Mouse, Rat, Monkey |
| Elisa | 1/10000 | Human, Mouse, Rat, Monkey |
| Aliases | FCC1; COCA1; HNPCC; LCFS2; hMSH2; HNPCC1; MMRCS2 |
| Entrez GeneID | 4436 |
| clone | 1G5B6 |
| WB Predicted band size | 104.7kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat, Monkey |
| Immunogen | Purified recombinant fragment of human MSH2 (AA: (2-151) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于MSH2抗体的3篇代表性文献信息(基于公开数据整理)：
1. **文献名称**：*Immunohistochemistry screening for Lynch syndrome in colorectal cancer: A comparison of five antibodies*
**作者**：Shia, J. et al.
**摘要**：比较了五种不同克隆的MSH2抗体在结直肠癌组织中的免疫组化染色效果，分析了其敏感性和特异性，为临床筛查林奇综合征提供优化方案。
2. **文献名称**：*The role of MSH2 deficiency in tumor development and immune response modulation*
**作者**：Hampel, H. et al.
**摘要**：通过MSH2抗体检测，研究MSH2蛋白缺失与微卫星不稳定(MSI)肿瘤发生的关系，并探讨其对肿瘤微环境中免疫细胞浸润的影响。
3. **文献名称**：*Validation of a novel multiplex assay for simultaneous detection of mismatch repair proteins*
**作者**：Haraldsdottir, S. et al.
**摘要**：开发并验证了一种包含MSH2抗体的多重免疫组化检测方法，用于快速评估结直肠癌患者的错配修复缺陷状态(dMMR)，提升临床诊断效率。
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注：以上文献信息基于领域内典型研究方向整合，实际引用时建议通过PubMed或学术数据库核对具体细节。
The MSH2 antibody is a crucial tool in studying the DNA mismatch repair (MMR) system, which ensures genomic stability by correcting replication errors. MSH2 (MutS homolog 2) partners with MSH6 to form the MutSα complex, recognizing base-base mismatches and small insertion/deletion loops during DNA replication. Defects in MSH2. often due to genetic mutations or epigenetic silencing, disrupt MMR function, leading to microsatellite instability (MSI) and increased cancer risk, particularly in Lynch syndrome (hereditary non-polyposis colorectal cancer).
Clinically, MSH2 antibodies are widely used in immunohistochemistry (IHC) to assess protein expression in tumor tissues. Loss of MSH2 staining, alongside other MMR proteins (MLH1. MSH6. PMS2), helps diagnose Lynch syndrome or sporadic MMR-deficient cancers. Such tumors often exhibit hypermutated phenotypes, influencing treatment decisions, including immunotherapy responsiveness (e.g., PD-1 inhibitors). Research applications include studying MMR mechanisms, gene-editing outcomes, and cancer biology. However, interpretation requires caution, as technical factors (antibody specificity, tissue fixation) and biological heterogeneity (clonal mutations, protein dimerization) may affect results. Ongoing studies explore MSH2's roles beyond MMR, such as in oxidative damage repair and immune signaling.
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