| WB | 1/500 - 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 1/200 - 1/1000 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | CMT2DD; HOMGSMR2 |
| Entrez GeneID | 476 |
| clone | 3C1F3 |
| WB Predicted band size | 112.8kDa |
| Host/Isotype | Mouse IgG2a |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human ATP1A1 (AA: 153-288) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
+ +
以下是与ATP1A1抗体相关的3篇参考文献示例(注：文献为虚构示例，仅作格式参考)：
1. **文献名称**：*Autoantibodies against ATP1A1 in Preeclampsia: Pathogenic Implications*
**作者**：Zhang Y, et al.
**摘要**：通过ELISA和免疫印迹法检测子痫前期患者血清，发现ATP1A1自身抗体水平显著升高，可能通过抑制钠钾泵活性导致血管内皮细胞功能障碍，提示其参与疾病发生机制。
2. **文献名称**：*ATP1A1 Overexpression as a Prognostic Marker in Colorectal Cancer*
**作者**：Smith A, et al.
**摘要**：利用免疫组化分析结肠癌组织，发现ATP1A1蛋白高表达与患者肿瘤转移和生存期缩短相关，表明其可作为潜在预后生物标志物。
3. **文献名称**：*ATP1A1 Antibody-Based Targeting in Chemoresistant Ovarian Cancer*
**作者**：Lee S, et al.
**摘要**：研究证实ATP1A1在顺铂耐药卵巢癌细胞中异常高表达，使用单克隆抗体阻断其功能可增强化疗敏感性，为克服耐药性提供新策略。
4. **文献名称**：*Neuronal ATP1A1 Localization and Epileptic Activity*
**作者**：Johnson R, et al.
**摘要**：通过免疫荧光和Western blot，发现ATP1A1在癫痫模型小鼠海马神经元膜上的分布改变，提示其异常表达可能影响神经元兴奋性及癫痫发作。
(注：以上文献为模拟内容，实际引用需查询真实数据库如PubMed。)
ATP1A1 antibody targets the alpha-1 subunit of the sodium-potassium ATPase (Na+/K+ ATPase), a critical transmembrane enzyme responsible for maintaining electrochemical gradients across cell membranes. This subunit, encoded by the *ATP1A1* gene, is part of the P-type ATPase family and plays a central role in active transport by hydrolyzing ATP to exchange intracellular sodium ions for extracellular potassium ions (3:2 ratio), essential for cellular homeostasis, nerve impulse transmission, and muscle contraction.
ATP1A1 is ubiquitously expressed, with high levels in the kidney, brain, and excitable tissues. Its dysfunction is linked to pathologies like hypertension, neurological disorders, and renal tubulopathies. Autoantibodies against ATP1A1 are implicated in autoimmune conditions, including neuromyotonia and certain forms of hypertension, where they disrupt ion transport, leading to hyperexcitability or electrolyte imbalance.
In research, ATP1A1 antibodies are widely used in techniques (e.g., Western blot, immunohistochemistry) to study protein expression, localization, and regulatory mechanisms. They also aid in exploring cancer biology, as altered Na+/K+ ATPase activity is associated with tumor progression and chemoresistance. Commercial antibodies are typically validated for specificity against the conserved regions of the α1 subunit, though cross-reactivity with other isoforms (α2-α4) must be considered.
Overall, ATP1A1 antibodies serve as vital tools for dissecting ion transport physiology and disease mechanisms, bridging basic research and clinical diagnostics.
×
