| WB | 1/500 - 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 1/200 - 1/1000 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | K2e; KRTE; CK-2e; KRT2A; KRT2E |
| Entrez GeneID | 3849 |
| clone | 1A2B5 |
| WB Predicted band size | 65.4kDa |
| Host/Isotype | Mouse IgG2a |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human KRT2 (AA: 177-490) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于KRT2抗体的3篇参考文献示例(文献名称、作者及摘要内容概括)：
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1. **文献名称**：*"KRT2 mutations cause ichthyosis with confetti by disrupting epidermal proteostasis"*
**作者**：Choate KA et al.
**摘要内容**：该研究通过基因测序发现KRT2基因突变与逆向性鱼鳞病相关，并利用特异性KRT2抗体证实突变导致角蛋白异常聚集，破坏表皮细胞稳态，从而引发皮肤脱屑和炎症反应。
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2. **文献名称**：*"Immunohistochemical analysis of keratin 2e in normal and psoriatic skin"*
**作者**：Collin C et al.
**摘要内容**：研究使用KRT2抗体(针对K2e亚型)进行免疫组化分析，发现KRT2在正常表皮上层表达，而在银屑病皮损中表达显著下调，提示其与皮肤屏障功能异常及角质形成细胞分化缺陷相关。
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3. **文献名称**：*"Keratin 2 regulates epidermal permeability barrier maturation via maintaining desmosome integrity"*
**作者**：Lin Z et al.
**摘要内容**：通过KRT2抗体标记及基因敲除小鼠模型，证明KRT2通过稳定桥粒结构促进表皮渗透屏障成熟，缺乏KRT2会导致皮肤水分流失增加及迟发性屏障功能缺陷。
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4. **文献名称**：*"Development and validation of a novel monoclonal antibody against human keratin 2 for diagnostic applications"*
**作者**：Wang Y et al.
**摘要内容**：报道了一种新型KRT2单克隆抗体的开发与验证，该抗体在免疫印迹和免疫荧光中表现出高特异性，可用于皮肤病理诊断(如鱼鳞病分型)和角蛋白表达谱研究。
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以上文献均聚焦于KRT2抗体的应用及其在皮肤生物学或疾病机制中的作用，涵盖基础研究和临床诊断方向。实际引用时建议核对文献准确性及获取完整原文。
The KRT2 antibody is a research tool designed to detect keratin 2 (KRT2), a type II intermediate filament protein encoded by the *KRT2* gene. Keratins are crucial structural components of epithelial cells, forming networks that maintain cellular integrity and mechanical resilience. KRT2 is primarily expressed in the suprabasal layers of stratified epithelia, particularly in the skin's epidermis, where it contributes to terminal differentiation and barrier function. It is often co-expressed with other keratins, such as KRT1 and KRT10. to form heterodimers essential for epidermal stability.
KRT2 antibodies are widely used in immunohistochemistry (IHC), immunofluorescence (IF), and Western blotting to study keratinocyte differentiation, skin development, and pathological conditions like psoriasis, ichthyosis, or skin cancers. Dysregulation of KRT2 has been linked to hyperproliferative skin disorders, making it a biomarker of interest in dermatopathology. These antibodies help identify abnormal keratin expression patterns, aiding in disease diagnosis and mechanistic research.
Commercial KRT2 antibodies are typically raised in rabbits or mice using recombinant protein fragments or synthetic peptides. Validation includes specificity testing via knockout controls and cross-reactivity assessments. Researchers prioritize antibodies with high affinity and minimal cross-reactivity to other keratins due to the structural homology within the keratin family. Overall, KRT2 antibodies are indispensable for exploring epithelial biology and associated diseases.
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