| WB | 1/500 - 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 1/200 - 1/1000 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | HD1; RPD3; KDAC1; GON-10; RPD3L1 |
| Entrez GeneID | 3065 |
| clone | 2A1D3 |
| WB Predicted band size | 55.1kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Purified recombinant fragment of human HDAC1 (AA: 321-482) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是3篇关于HDAC1抗体的参考文献及其摘要概括：
1. **"Histone deacetylases specifically down-regulate p53-dependent gene activation"**
- **作者**: Luo et al. (2000)
- **摘要**: 该研究使用HDAC1抗体通过免疫共沉淀(Co-IP)和染色质免疫沉淀(ChIP)技术，揭示了HDAC1与肿瘤抑制蛋白p53的相互作用，证明HDAC1通过去乙酰化抑制p53介导的基因转录调控。
2. **"HDAC1 regulates pluripotency and lineage specific transcriptional networks in embryonic and trophoblast stem cells"**
- **作者**: Dovey et al. (2010)
- **摘要**: 通过HDAC1抗体的Western blot和免疫荧光分析，研究发现HDAC1在胚胎干细胞和滋养层干细胞中调控多能性基因网络，并参与细胞分化的表观遗传调控。
3. **"A systematic evaluation of antibodies targeting chromatin modification enzymes"**
- **作者**: Egelhofer et al. (2011)
- **摘要**: 该文献系统评估了包括HDAC1抗体在内的多种表观遗传相关抗体，通过ChIP-seq和免疫沉淀验证了HDAC1抗体的特异性及在染色质结合分析中的应用可靠性。
这些文献涵盖了HDAC1抗体在蛋白质互作、干细胞分化及抗体验证中的关键应用场景。
HDAC1 (Histone Deacetylase 1) is a key enzyme involved in epigenetic regulation, primarily responsible for removing acetyl groups from histone proteins, leading to chromatin condensation and transcriptional repression. As a member of the Class I HDAC family, it plays critical roles in cell cycle progression, differentiation, apoptosis, and DNA repair by modulating chromatin structure and interacting with transcription factors. Dysregulation of HDAC1 is implicated in various diseases, including cancers, neurodegenerative disorders, and inflammatory conditions, making it a significant therapeutic target.
HDAC1 antibodies are essential tools for studying its expression, localization, and function in biological systems. These antibodies are widely used in techniques like Western blotting, immunohistochemistry (IHC), immunoprecipitation (IP), and chromatin immunoprecipitation (ChIP) to detect HDAC1 protein levels, assess post-translational modifications, or map its binding sites on DNA. Specific HDAC1 antibodies are validated for cross-reactivity and selectivity against other HDAC isoforms (e.g., HDAC2. HDAC3) to ensure experimental accuracy. Researchers also utilize these antibodies to explore HDAC1's role in disease mechanisms or evaluate the efficacy of HDAC inhibitors in preclinical models. Commercial HDAC1 antibodies are typically raised against epitopes within conserved regions of the protein, often available as monoclonal or polyclonal formats with compatibility across human, mouse, and rat samples. Proper validation using knockout controls or siRNA knockdown is recommended to confirm antibody specificity.
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