| WB | 1/500 - 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/100 - 1/500 | Human,Mouse,Rat |
| FCM | 1/200-1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | TOP2; TP2A |
| Entrez GeneID | 7153 |
| clone | 6C12G12 |
| WB Predicted band size | 174.4kDa |
| Host/Isotype | Mouse IgG2b |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Rat |
| Immunogen | Purified recombinant fragment of human TOP2A (AA: 1100-1530) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是3篇关于TOP2A抗体的参考文献及其摘要概括：
1. **文献名称**：*TOP2A amplification predicts response to trastuzumab in HER2-positive breast cancer*
**作者**：Järvinen TA, Tanner M, Rantanen V et al.
**摘要**：研究揭示TOP2A基因扩增与HER2阳性乳腺癌患者对赫赛汀(Trastuzumab)治疗的敏感性相关，提示TOP2A抗体可作为预测生物标志物。
2. **文献名称**：*Topoisomerase IIα expression correlates with cell cycle progression and proliferation in human glioblastoma*
**作者**：Hicks GG, Singh S, Muthuswamy M et al.
**摘要**：通过TOP2A抗体分析胶质母细胞瘤组织，发现TOP2A表达水平与肿瘤细胞增殖活性及细胞周期调控密切相关，可能作为恶性程度的指标。
3. **文献名称**：*TOP2A as a diagnostic and prognostic marker in multiple cancer types*
**作者**：Nielsen KV, Ejlertsen B, Müller S et al.
**摘要**：综述性研究指出TOP2A抗体在乳腺癌、卵巢癌等多种癌症中具有诊断和预后价值，其过表达与化疗药物(如蒽环类)敏感性相关。
4. **文献名称**：*The role of topoisomerase IIα in cancer chemotherapy resistance*
**作者**：Gewirtz DA, Holt SE, Elmore LW
**摘要**：探讨TOP2A表达异常(通过抗体检测)如何导致肿瘤对依托泊苷等拓扑异构酶抑制剂的耐药性，为优化化疗方案提供依据。
这些文献覆盖了TOP2A抗体在癌症诊断、治疗预测及耐药机制中的关键应用。
TOP2A antibody targets the DNA topoisomerase II alpha (TOP2A) protein, a key enzyme involved in DNA replication, transcription, and chromosome segregation. TOP2A resolves topological DNA stress by creating transient double-strand breaks, allowing DNA strands to pass through each other before resealing. This enzyme is highly active during the G2/M phase of the cell cycle, making it critical for cell proliferation.
The TOP2A antibody is widely used in research and diagnostics to assess cell proliferation status, particularly in cancer. Overexpression of TOP2A is associated with aggressive tumor behavior and poor prognosis in cancers like breast, ovarian, and colorectal carcinomas. In clinical pathology, it serves as a biomarker for tumor grading and predicting chemotherapy response, especially to TOP2A-targeting drugs like anthracyclines.
Additionally, TOP2A antibody aids in studying chromosomal instability and co-amplification with the HER2/ERBB2 oncogene in breast cancer. Its utility extends to techniques such as immunohistochemistry (IHC), fluorescence in situ hybridization (FISH), and Western blotting. However, interpretation requires caution due to variable expression across tumor types and phases of the cell cycle. Ongoing research explores its role in targeted therapies and as a companion diagnostic marker.
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