| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DFFB |
| Uniprot No | O76075 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-338aa |
| 氨基酸序列 | MLQKPKSVKLRALRSPRKFGVAGRSCQEVLRKGCLRFQLPERGSRLCLYEDGTELTEDYFPSVPDNAELVLLTLGQAWQGYVSDIRRFLSAFHEPQVGLIQAAQQLLCDEQAPQRQRLLADLLHNVSQNIAAETRAEDPPWFEGLESRFQSKSGYLRYSCESRIRSYLREVSSYPSTVGAEAQEEFLRVLGSMCQRLRSMQYNGSYFDRGAKGGSRLCTPEGWFSCQGPFDMDSCLSRHSINPYSNRESRILFSTWNLDHIIEKKRTIIPTLVEAIKEQDGREVDWEYFYGLLFTSENLKLVHIVCHKKTTHKLNCDPSRIYKPQTRLKRKQPVRKRQ |
| 预测分子量 | 45.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于DFFB重组蛋白的3篇示例文献(内容为模拟概括，建议通过学术数据库核实)：
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1. **文献名称**：*Expression and Purification of Recombinant DFFB in Escherichia coli for Apoptosis Studies*
**作者**：Smith J, et al.
**摘要**：本研究报道了通过大肠杆菌表达系统高效生产重组DFFB蛋白的方法，优化了纯化步骤并验证其诱导DNA断裂的体外活性，为细胞凋亡机制研究提供了工具。
2. **文献名称**：*Structural Analysis of DFFB Reveals Key Domains for Caspase-3 Activation*
**作者**：Chen L, Wang H.
**摘要**：通过X射线晶体学解析了重组DFFB蛋白的结构，揭示了其与Caspase-3相互作用的结合位点，阐明了DFFB在凋亡信号通路中的分子机制。
3. **文献名称**：*Functional Characterization of Recombinant DFFB in Neurodegenerative Disease Models*
**作者**：Kim S, et al.
**摘要**：利用重组DFFB蛋白研究其在神经退行性疾病中的调控作用，发现DFFB异常激活可加剧神经元DNA损伤，提示其作为潜在治疗靶点。
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**建议**：实际文献可通过PubMed、Google Scholar等平台以关键词"DFFB recombinant protein"或"DNA fragmentation factor beta expression"检索，并筛选近5年研究以获取最新进展。
**Background of DFFB Recombinant Protein**
DFFB (DNA Fragmentation Factor Subunit Beta), also known as DFF40 or caspase-activated DNase (CAD), is a key protein involved in apoptosis. It functions as a nuclease responsible for digesting genomic DNA into oligonucleosomal fragments, a hallmark of programmed cell death. DFFB is typically stored in the cytoplasm as an inactive heterodimer complex with its inhibitor subunit, DFFA (or DFF45). During apoptosis, caspase-3 cleaves DFFA, releasing DFFB to translocate into the nucleus and execute DNA fragmentation.
Recombinant DFFB protein is produced using genetic engineering techniques, often expressed in *E. coli* or mammalian cell systems. This allows large-scale production of highly pure, biologically active DFFB for research applications. The recombinant form is crucial for studying apoptosis mechanisms, as it enables controlled in vitro experiments to dissect DFFB's enzymatic activity, regulatory interactions (e.g., with DFFA or ICAD), and its role in diseases like cancer or neurodegenerative disorders.
Researchers utilize DFFB recombinant protein to screen potential therapeutic agents targeting apoptosis pathways, investigate DNA damage responses, or develop diagnostic tools. Its structural and functional characterization has also advanced understanding of nuclease regulation, offering insights into designing modulators for cell death-related therapies. Overall, DFFB recombinant protein serves as a vital tool in apoptosis research and drug discovery.
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