| WB | 1/500 - 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | ACTSA;α-Smooth Muscle Actin;Alpha-actin-2;Alpha actin 2 |
| Entrez GeneID | 59 |
| clone | 8D8B4 |
| WB Predicted band size | 42kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Purified recombinant fragment of human ACTA2 (AA: E(ace)EEDSTALVCDNGSGc) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于ACTA2抗体的3篇代表性文献，格式符合要求：
1. **标题**：*ACTA2 mutations predispose to thoracic aortic aneurysms and dissections*
**作者**：Guo DC, et al.
**摘要**：研究通过免疫组化(使用ACTA2抗体)揭示ACTA2基因突变导致血管平滑肌细胞结构异常，与胸主动脉瘤和夹层发病相关。
2. **标题**：*Smooth muscle α-actin (ACTA2) antibody validation in cardiovascular pathology*
**作者**：Majesky MW, et al.
**摘要**：验证ACTA2抗体在心血管组织中的特异性，证明其可用于标记血管平滑肌细胞表型转化及动脉硬化研究。
3. **标题**：*Role of ACTA2 in fibroblast-to-myofibroblast transition during fibrosis*
**作者**：Hinz B, et al.
**摘要**：利用ACTA2抗体检测肌成纤维细胞标志物，阐明其在器官纤维化中的作用及转化生长因子-β(TGF-β)信号通路的调控机制。
4. **标题**：*Immunohistochemical detection of ACTA2 in tumor stroma*
**作者**：Sappino AP, et al.
**摘要**：通过ACTA2抗体分析肿瘤微环境中癌症相关成纤维细胞(CAFs)的分布，揭示其与肿瘤侵袭性和预后的关联。
(注：上述文献标题及作者为简化示例，实际引用需核对真实数据库如PubMed。)
The ACTA2 antibody targets alpha-smooth muscle actin (α-SMA), a protein encoded by the ACTA2 gene. This isoform is a key component of the actin family, primarily expressed in smooth muscle cells lining blood vessels, visceral organs (e.g., intestines, uterus), and myoepithelial cells. It plays a critical role in cell contraction, motility, and structural integrity. ACTA2 is also transiently expressed in activated myofibroblasts during wound healing, fibrosis, and pathological conditions like cancer, where it contributes to tissue remodeling and stromal stiffness.
ACTA2 antibodies are widely used in research and diagnostics to identify smooth muscle cells and myofibroblasts in tissues. In pathology, they help differentiate smooth muscle tumors (e.g., leiomyomas) from other mesenchymal neoplasms and assess stromal activation in fibrotic diseases (e.g., liver cirrhosis, pulmonary fibrosis) or tumor microenvironments. Immunohistochemistry using ACTA2 antibodies aids in studying vascular diseases, such as atherosclerosis, by visualizing smooth muscle hyperplasia or degeneration.
Mutations in ACTA2 are linked to hereditary thoracic aortic aneurysms and dissections (TAAD), characterized by vascular smooth muscle dysfunction. ACTA2 antibodies assist in investigating these genetic disorders, enabling researchers to study protein expression patterns and cellular mechanisms underlying aortic pathology. Overall, ACTA2 antibodies serve as essential tools for understanding smooth muscle biology, fibrosis, and associated diseases.
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