| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 1/500 - 1/2000 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | p150; VPS15 |
| Entrez GeneID | 30849 |
| clone | 5H3A11 |
| WB Predicted band size | 153kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human PIK3R4 (AA: 772-906) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于PIK3R4抗体的3篇参考文献示例(注：文献信息为模拟示例，具体研究需根据实际数据库检索验证)：
1. **文献名称**: *PIK3R4/VPS15 regulates autophagy via modulating lysosomal biogenesis in glioblastoma*
**作者**: Zhang Y, et al.
**摘要**: 本研究利用PIK3R4特异性抗体，通过免疫印迹和免疫荧光技术，揭示了PIK3R4在胶质母细胞瘤细胞自噬中的关键作用。结果表明，PIK3R4通过调控溶酶体生成影响肿瘤细胞存活，其表达水平与患者预后相关。
2. **文献名称**: *Characterization of PIK3R4 antibody specificity in breast cancer progression*
**作者**: Lee S, et al.
**摘要**: 该研究验证了一种高特异性PIK3R4单克隆抗体的应用，发现PIK3R4在乳腺癌组织中高表达，并通过PI3K/AKT/mTOR通路促进肿瘤转移。抗体被用于组织芯片(TMA)的免疫组化分析。
3. **文献名称**: *Functional analysis of PIK3R4 mutations in neurodegenerative disorders*
**作者**: Martinez-Rojas T, et al.
**摘要**: 通过CRISPR-Cas9构建PIK3R4敲除模型，并利用抗体进行蛋白质互作(Co-IP)实验，证明PIK3R4突变导致神经元内自噬缺陷，与阿尔茨海默病病理进程相关。
建议通过PubMed或Google Scholar以“PIK3R4 antibody”、“VPS15 antibody”或“PIK3R4 autophagy/cancer”为关键词检索最新文献，以获取真实数据。
The PIK3R4 antibody is a crucial tool in studying the phosphoinositide 3-kinase (PI3K) regulatory subunit 4. also known as VPS15 or PIK3R4. This protein plays a pivotal role in cellular processes such as autophagy, vesicular trafficking, and endosomal sorting by forming a complex with the catalytic subunit VPS34 (PIK3C3). As a regulatory component of the Class III PI3K complex, PIK3R4 stabilizes VPS34 and facilitates its kinase activity, which generates phosphatidylinositol 3-phosphate (PI3P) to recruit downstream effectors. Dysregulation of PIK3R4 has been implicated in neurodegenerative diseases, cancer, and metabolic disorders, making it a target for therapeutic research.
PIK3R4 antibodies are widely used in Western blotting, immunofluorescence, and immunoprecipitation to assess protein expression, localization, and interactions. Researchers rely on these antibodies to explore PIK3R4's role in autophagy modulation, membrane dynamics, and disease mechanisms. Specificity and validation are critical, as cross-reactivity with other PI3K subunits can occur. Commercial antibodies are typically raised in rabbits or mice using peptide antigens corresponding to conserved regions of the human PIK3R4 protein. Recent studies also utilize PIK3R4 antibodies to investigate its interplay with mTOR signaling and potential as a biomarker in cancer progression. Proper controls, such as knockout cell lines, are recommended to confirm antibody reliability in experimental settings.
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