| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | CDM; HCAD; LCAD; H-CAD; L-CAD; NAG22 |
| Entrez GeneID | 800 |
| clone | 6F8D2 |
| WB Predicted band size | 93.2kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human CALD1 (AA: 26-207) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于CALD1抗体的3篇参考文献的简要信息：
1. **文献名称**：Caldesmon as a marker for smooth muscle differentiation in cancer
**作者**：Hirokawa Y, et al.
**摘要**：该研究利用CALD1抗体验证了CALD1在多种肿瘤中的表达，发现其可作为平滑肌分化标志物，并提示其过表达与肿瘤侵袭性相关。通过免疫组化分析胃癌及肉瘤样本，证实CALD1抗体在区分肿瘤亚型中的诊断价值。
2. **文献名称**：Role of caldesmon in cell migration and cytoskeletal remodeling
**作者**：Tang DD, et al.
**摘要**：通过CALD1特异性抗体进行Western blot和免疫荧光实验，揭示CALD1通过调控肌动蛋白-肌球蛋白相互作用影响细胞迁移。研究在血管内皮细胞模型中证明，CALD1磷酸化状态的变化直接影响细胞骨架动态。
3. **文献名称**：Antibody-based profiling of CALD1 isoforms in lung adenocarcinoma
**作者**：Chen X, et al.
**摘要**：开发了针对CALD1不同剪接变体的特异性抗体，结合质谱分析，发现高分子量CALD1(h-CALD1)在肺腺癌中的特异性高表达，并与患者不良预后显著相关。研究为CALD1异构体的功能差异提供了实验依据。
注：以上文献信息为示例性质，实际文献需通过PubMed或Web of Science等数据库检索确认。建议结合具体研究方向补充近年研究(如单细胞测序中CALD1抗体的应用或肿瘤微环境相关机制)。
The CALD1 antibody targets caldesmon, a cytoskeleton-associated protein encoded by the CALD1 gene. Caldesmon plays a critical role in regulating smooth muscle and non-muscle cell contraction by modulating actin-myosin interactions. It exists in two major isoforms: the high-molecular-weight (h-CaD), predominantly expressed in smooth muscle tissues, and the low-molecular-weight (l-CaD), found in non-muscle cells. Both isoforms function as actin-stabilizing proteins, influencing cell motility, adhesion, and cytoskeletal remodeling.
CALD1 antibodies are widely used in research to investigate caldesmon's involvement in physiological and pathological processes, such as vascular tone regulation, tumor progression, and metastasis. In cancer biology, caldesmon overexpression has been linked to enhanced cell migration and invasion in malignancies like breast and colorectal cancers, making it a potential biomarker for aggressiveness. These antibodies are employed in techniques like immunohistochemistry (IHC), Western blotting, and immunofluorescence to assess protein expression, localization, and post-translational modifications.
Additionally, CALD1 antibodies aid in studying vascular diseases, including hypertension and atherosclerosis, where smooth muscle dysfunction is implicated. Their specificity for distinguishing between h-CaD and l-CaD isoforms is crucial for understanding tissue-specific mechanisms. As caldesmon activity is regulated by phosphorylation via kinases like ERK, these tools also help dissect signaling pathways influencing cell contractility and proliferation. Overall, CALD1 antibodies serve as vital reagents in exploring cytoskeletal dynamics and their implications in health and disease.
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