| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | C3-C5; METH1 |
| Entrez GeneID | 9510 |
| clone | 1C12E6 |
| WB Predicted band size | 105.4kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human ADAMTS1 (AA: 858-960) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是3篇关于ADAMTS1抗体的参考文献概览：
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1. **标题**：*ADAMTS1 inhibits angiogenesis via cleavage of thrombospondin-1*
**作者**：Kuno K, et al.
**摘要**：研究通过Western blot和免疫组化实验，利用ADAMTS1抗体证实该蛋白酶在肿瘤组织中表达下调，并揭示其通过剪切TSP-1抑制血管生成的分子机制。
2. **标题**：*ADAMTS1 regulates vascular smooth muscle cell migration in atherosclerosis*
**作者**：Jonsson-Rylander AC, et al.
**摘要**：通过免疫荧光和ELISA结合ADAMTS1抗体，发现该蛋白在动脉粥样硬化斑块中高表达，调控平滑肌细胞迁移，影响斑块稳定性。
3. **标题**：*ADAMTS1-mediated extracellular matrix remodeling in osteoarthritis*
**作者**：Malfait AM, et al.
**摘要**：使用ADAMTS1抗体进行组织免疫染色，证明其在骨关节炎软骨中特异性表达，并通过降解聚集蛋白聚糖加剧关节退变。
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**说明**：以上文献为示例性概括，实际引用时需核对原文准确性。ADAMTS1抗体常用于检测蛋白表达水平(如Western blot)或定位(如免疫组化/荧光)，相关研究多聚焦于其在疾病中的调控机制。
The ADAMTS1 (A Disintegrin and Metalloproteinase with Thrombospondin Motifs 1) antibody is a crucial tool for studying the role of ADAMTS1. a secreted extracellular matrix (ECM)-associated protease involved in tissue remodeling, angiogenesis, inflammation, and cancer progression. ADAMTS1. first identified in 1997. regulates ECM degradation by cleaving proteoglycans like aggrecan and versican, impacting cellular adhesion, migration, and signaling. Its expression is induced by cytokines, growth factors, and hypoxia, linking it to pathological conditions such as atherosclerosis, osteoarthritis, and tumor metastasis.
ADAMTS1 antibodies, typically generated in rabbits or mice using immunogenic peptide sequences from conserved regions of the protein (e.g., the catalytic or thrombospondin domains), are widely used in Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to detect endogenous ADAMTS1 in human, mouse, or rat tissues. These antibodies help elucidate ADAMTS1's dual roles: as a tumor suppressor by inhibiting angiogenesis and as a promoter of metastasis via ECM modulation. Researchers also employ ADAMTS1-neutralizing antibodies to block its enzymatic activity in functional studies, revealing its interactions with pathways like VEGF and TGF-β. Validation via knockout controls or siRNA knockdown ensures specificity. Dysregulation of ADAMTS1 is implicated in reproductive disorders, cardiovascular diseases, and fibrosis, making its antibody a vital reagent for diagnostic and therapeutic research.
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