| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ALDH1B1 |
| Uniprot No | P30837 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 167-264aa |
| 氨基酸序列 | SRVISQPDQTLEILMSELDPAVMDQFYMKDGVTAKDVTRESGIRDLIPGSVIDATMFNPCGYSMNGMKSDGTYWTIHITPEPEFSYVSFETNLSQTSY |
| 预测分子量 | 47.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **"ALDH1B1: A mitochondrial dehydrogenase associated with colon cancer stem cells"**
- **作者**: Chen Y, et al.
- **摘要**: 研究通过重组表达ALDH1B1蛋白，验证其在线粒体代谢中的功能，并发现其高表达与结肠癌干细胞特性及化疗耐药性相关。
2. **"Structural and functional characterization of human ALDH1B1. a mitochondrial isoform of aldehyde dehydrogenase"**
- **作者**: Jackson B, et al.
- **摘要**: 利用重组ALDH1B1蛋白进行晶体结构解析和酶动力学分析，揭示其底物特异性及与糖尿病潜在关联的分子机制。
3. **"ALDH1B1 polymorphisms influence drug metabolism and toxicity in vitro"**
- **作者**: Singh S, et al.
- **摘要**: 通过重组ALDH1B1蛋白体外实验，证明其遗传多态性影响对特定药物(如环磷酰胺)的代谢活性，提示个体化用药的潜在价值。
4. **"Role of ALDH1B1 in pancreatic β-cell function and glucose homeostasis"**
- **作者**: Kim H, et al.
- **摘要**: 利用重组蛋白及基因敲除模型，发现ALDH1B1通过调控线粒体氧化应激维持胰岛β细胞功能，为糖尿病治疗提供新靶点。
ALDH1B1 (Aldehyde Dehydrogenase 1 Family Member B1) is a mitochondrial enzyme belonging to the aldehyde dehydrogenase superfamily, which plays critical roles in detoxifying endogenous and exogenous aldehydes by oxidizing them to carboxylic acids. This 56 kDa protein is encoded by the ALDH1B1 gene located on chromosome 9p13.3 in humans. It shares structural homology with other ALDH isoforms, featuring a conserved catalytic domain and NAD+-binding site. ALDH1B1 is primarily expressed in metabolically active tissues, including the intestines, liver, and pancreas, where it contributes to cellular redox balance and metabolic homeostasis.
Research highlights its involvement in multiple biological processes, such as retinoic acid synthesis, mitochondrial function, and stem cell regulation. Dysregulation of ALDH1B1 has been linked to pathological conditions, including cancer, diabetes, and alcohol-related disorders. For instance, elevated ALDH1B1 expression is observed in colorectal cancer, where it promotes tumorigenesis by enhancing reactive oxygen species (ROS) production and activating Wnt/β-catenin signaling. Conversely, its downregulation in pancreatic β-cells is associated with impaired glucose-stimulated insulin secretion, suggesting a role in diabetes progression.
Recombinant ALDH1B1 protein, produced via heterologous expression systems (e.g., E. coli or mammalian cells), enables functional studies to dissect its enzymatic activity, substrate specificity, and interaction partners. Purification often involves affinity tags (e.g., His-tag) followed by biochemical validation. Its applications span enzyme kinetics, inhibitor screening, antibody development, and disease modeling. As a potential therapeutic target, understanding ALDH1B1's mechanisms in health and disease remains a focus for drug discovery and biomarker research.
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