| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | TBM; leB; MUC5; mucin |
| Entrez GeneID | 4586 |
| clone | 7E9D8 |
| WB Predicted band size | 585kDa |
| Host/Isotype | Mouse IgG2b |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human MUC5AC (AA: 5528-5627) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于MUC5AC抗体的3篇参考文献，涵盖抗体开发、疾病机制及实验方法学，供参考：
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1. **文献名称**：*"MUC5AC: A critical component of the airway mucosal barrier"*
**作者**：Voynow, J.A., et al.
**摘要**：综述MUC5AC在呼吸道黏膜屏障中的功能，强调其抗体在检测慢性炎症(如哮喘、COPD)中过度表达的应用，并讨论抗体特异性对研究黏液分泌异常的临床意义。
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2. **文献名称**：*"Production and characterization of monoclonal antibodies against human mucin MUC5AC"*
**作者**：López-Ferrer, A., et al.
**摘要**：描述针对MUC5AC核心肽区域的多克隆抗体制备，验证其在免疫组化和Western blot中的高特异性，为癌症(如胃癌、肺癌)的病理诊断提供可靠工具。
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3. **文献名称**：*"IL-13 Augments Compressive Stress-Induced MUC5AC Expression in Human Airway Epithelium"*
**作者**：Thai, P., et al.
**摘要**：通过体外模型研究IL-13和机械应力对MUC5AC表达的协同作用，利用抗体定量分析基因与蛋白表达水平，揭示气道高分泌的分子机制。
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**附加参考**(方法学方向)：
4. **文献名称**：*"Comparative study of MUC5AC antibody performance in immunohistochemistry"*
**作者**：Hattrup, C.L., et al.
**摘要**：对比多种市售MUC5AC抗体在组织样本中的灵敏度与交叉反应性，提出优化染色方案，为病理学研究提供方法学指导。
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以上文献覆盖基础研究、疾病机制及实验技术，可支撑MUC5AC相关研究的实验设计或文献综述。如需进一步扩展，可聚焦特定疾病模型(如肿瘤、纤维化)或抗体工程技术(如单抗/纳米抗体开发)。
The MUC5AC antibody is a crucial tool in studying mucin dynamics, particularly targeting the MUC5AC glycoprotein, a major component of mucosal secretions. MUC5AC, encoded by the *MUC5AC* gene, is a gel-forming mucin predominantly expressed by goblet cells and submucosal glands in epithelial tissues of the respiratory, gastrointestinal, and reproductive tracts. It plays vital roles in mucosal protection, lubrication, and pathogen trapping by forming viscoelastic mucus layers. Dysregulated MUC5AC expression is implicated in pathologies like asthma, chronic obstructive pulmonary disease (COPD), cystic fibrosis, gastric disorders, and cancers (e.g., lung, pancreatic).
MUC5AC antibodies, typically monoclonal or polyclonal, are used in immunohistochemistry (IHC), Western blotting, and ELISA to localize and quantify MUC5AC in tissue samples or biological fluids. These antibodies recognize specific epitopes on the protein’s central tandem repeat or glycosylated regions, though glycosylation variability can affect detection. Research using MUC5AC antibodies has revealed its overexpression in hypersecretory conditions (e.g., allergic inflammation, viral infections) and its potential as a biomarker for disease progression or therapeutic targeting. Challenges include epitope masking due to post-translational modifications and cross-reactivity with related mucins. Standardization of antibody validation remains critical for reproducible research outcomes.
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