| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GPR132 |
| Uniprot No | Q9UNW8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 311-380aa |
| 氨基酸序列 | ATDHSRQEVSRIHKGWKEWSMKTDVTRLTHSRDTEELQSPVALADHYTFSRPVHPPGSPCPAKRLIEESC |
| 预测分子量 | 39.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于GPR132重组蛋白的模拟参考文献示例(仅供参考，建议通过学术数据库核实真实文献)：
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1. **文献名称**: *"GPR132 Recombinant Protein Modulates Macrophage Polarization in Inflammatory Responses"*
**作者**: Lin, Y. et al.
**摘要**: 本研究通过大肠杆菌表达系统制备了重组GPR132蛋白，并验证其在巨噬细胞中的功能。实验表明，重组GPR132通过调控cAMP信号通路促进M1型巨噬细胞极化，提示其在炎症性疾病中的潜在作用。
2. **文献名称**: *"Structural Characterization of Recombinant GPR132 and Its pH-Sensing Mechanism"*
**作者**: Hebert, T.E. et al.
**摘要**: 利用哺乳动物细胞表达系统纯化GPR132重组蛋白，结合冷冻电镜解析其三维结构。研究发现GPR132的胞外域对pH变化敏感，可能在肿瘤微环境中介导细胞迁移和侵袭。
3. **文献名称**: *"Recombinant GPR132 as a Therapeutic Target in Breast Cancer Metastasis"*
**作者**: Zhang, Q. et al.
**摘要**: 通过重组GPR132蛋白体外实验，证实其与溶血磷脂酸(LPA)的相互作用可激活下游MAPK通路，促进乳腺癌细胞转移。研究为靶向GPR132的癌症治疗提供了分子依据。
4. **文献名称**: *"Functional Analysis of GPR132 in Lipid Metabolism Using Recombinant Protein Models"*
**作者**: Obinata, H. et al.
**摘要**: 构建GPR132重组蛋白并用于配体筛选，发现其特异性结合氧化脂质衍生物(如9-HSA)，调节巨噬细胞脂质代谢和炎症反应，揭示了GPR132在动脉粥样硬化中的潜在机制。
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**注意**：以上内容为基于领域知识的模拟生成，实际文献需通过PubMed、Web of Science等平台检索关键词(如“GPR132 recombinant”“GPR132 expression”“GPR132 signaling”)获取。真实研究可能涉及重组蛋白的表达系统(如HEK293细胞)、功能验证(如配体结合、信号通路分析)或疾病模型应用。
GPR132 (G Protein-Coupled Receptor 132), also known as G2A, is a member of the proton-sensing G protein-coupled receptor (GPCR) family. It plays a critical role in cellular responses to lipid mediators and acidic microenvironmental conditions. Initially identified as a stress-inducible receptor, GPR132 is involved in immune regulation, inflammation, and metabolic processes. Structurally, it contains seven transmembrane domains characteristic of GPCRs and signals primarily through Gαi/o and Gα12/13 pathways, modulating downstream effectors like cAMP, MAPK, and Rho GTPases.
Biologically, GPR132 acts as a lipid sensor, responding to oxidized free fatty acids (e.g., 9-HODE) and lysophosphatidylcholine (LPC), linking metabolic stress to immune cell activation. It regulates macrophage polarization, influencing pro-inflammatory (M1) versus anti-inflammatory (M2) phenotypes in tumor microenvironments and atherosclerosis. Studies also associate GPR132 with cancer progression, where its expression in tumor-associated macrophages may promote angiogenesis and metastasis. In cardiovascular diseases, it contributes to foam cell formation by enhancing oxidized LDL uptake.
Recombinant GPR132 protein is engineered for in vitro studies to investigate receptor-ligand interactions, signaling mechanisms, and drug discovery. Typically produced in mammalian expression systems (e.g., HEK293 cells) to ensure proper post-translational modifications, the recombinant protein retains native conformational epitopes and functionality. Purification methods often involve affinity chromatography using tags like His or FLAG. Quality control includes SDS-PAGE, Western blotting, and functional assays (e.g., ligand-binding tests using radiolabeled compounds or fluorescent probes).
Research applications span structural biology (crystallography), high-throughput screening for therapeutic modulators, and mechanistic studies of GPR132’s role in disease pathways. Its dual role as a metabolic and immune checkpoint makes it a potential target for treating cancers, chronic inflammation, and metabolic disorders.
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