| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/200 - 1/1000 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | RIGI; RIG-I; RLR-1; SGMRT2 |
| Entrez GeneID | 23586 |
| clone | 4G1B6 |
| WB Predicted band size | 106.6kDa |
| Host/Isotype | Mouse IgM |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human DDX58 (AA: 789-925) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是3篇涉及DDX58(RIG-I)抗体的参考文献摘要：
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1. **Title**: *Structural basis for dsRNA recognition and antiviral response by RIG-I*
**Authors**: Jiang X, et al.
**Summary**: 本研究解析了RIG-I识别病毒双链RNA的分子机制，并利用特异性DDX58抗体验证其在细胞内的定位变化。抗体用于免疫沉淀实验，证实病毒感染后RIG-I的构象改变促进下游MAVS信号激活。
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2. **Title**: *Development of a monoclonal antibody specific for RIG-I reveals its dynamic expression in antiviral immunity*
**Authors**: Sato S, et al.
**Summary**: 研究者开发了一种高特异性抗人DDX58单克隆抗体，通过Western blot和免疫荧光验证其灵敏度。该抗体成功应用于检测病毒感染的细胞中RIG-I表达上调，为临床样本检测提供工具。
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3. **Title**: *RIG-I-like receptor signaling in autoimmune hepatitis*
**Authors**: Wang Y, et al.
**Summary**: 文章探讨RIG-I在自身免疫性肝炎中的作用，使用抗DDX58抗体进行组织染色，发现患者肝细胞中RIG-I异常激活与炎症因子释放相关，提示其作为潜在治疗靶点。
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这些文献展示了DDX58抗体在机制研究、诊断工具开发和疾病关联分析中的关键应用。
DDX58. also known as retinoic acid-inducible gene I (RIG-I), is a cytosolic RNA helicase critical in innate antiviral immunity. It acts as a pattern recognition receptor (PRR) that detects viral RNA, particularly short double-stranded RNA (dsRNA) or uncapped 5′-triphosphate single-stranded RNA, triggering downstream signaling to produce type I interferons (IFNs) and proinflammatory cytokines. Structurally, DDX58 contains two N-terminal caspase activation and recruitment domains (CARDs), a central DExD/H-box helicase domain, and a C-terminal regulatory domain. Upon RNA binding, DDX58 undergoes conformational changes, enabling CARD-mediated interaction with mitochondrial antiviral-signaling protein (MAVS) to activate IRF3/7 and NF-κB pathways.
Antibodies targeting DDX58 are essential tools for studying its expression, localization, and function in viral infections, autoimmune diseases, and cancer. They are widely used in techniques like Western blot, immunofluorescence, immunoprecipitation, and flow cytometry. Specific antibodies can distinguish between inactive and activated states of DDX58. aiding in research on its post-translational modifications (e.g., ubiquitination) or mutations linked to immune dysregulation. Commercially available monoclonal and polyclonal antibodies vary in species reactivity (human, mouse, etc.) and applications, requiring validation for experimental contexts. Dysregulation of DDX58 is implicated in conditions like Aicardi-Goutières syndrome and certain cancers, making its antibodies valuable for diagnostic and therapeutic exploration.
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