| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | CR; CAL2; CAB29 |
| Entrez GeneID | 794 |
| clone | 7F12G9 |
| WB Predicted band size | 31.5kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human CALB2 (AA: 172-271) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是3篇与CALB2(Calbindin 2/Calretinin)抗体相关的参考文献，按研究领域分类简要概括：
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1. **文献名称**：*Calretinin: A gene for a novel calcium-binding protein expressed principally in neurons*
**作者**：Schwaller, B. et al.
**摘要**：该研究首次克隆并鉴定了CALB2(Calretinin)基因，证实其编码的钙结合蛋白在哺乳动物中枢神经系统(如海马、皮层)的特定神经元亚群中高表达，并开发了特异性抗体用于神经元标记。
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2. **文献名称**：*Loss of calretinin-immunoreactive neurons in the hippocampus in Alzheimer's disease*
**作者**：Hof, P.R. et al.
**摘要**：通过CALB2抗体标记，研究发现阿尔茨海默病患者海马区Calretinin阳性神经元数量显著减少，提示这类神经元可能对淀粉样蛋白病理敏感，为疾病机制提供新线索。
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3. **文献名称**：*Selective vulnerability of calretinin-containing hippocampal interneurors in epilepsy*
**作者**：Tóth, K. et al.
**摘要**：利用CALB2抗体结合免疫组化，揭示癫痫模型中Calretinin阳性中间神经元易损性增加，其功能异常可能导致网络兴奋性失衡，为癫痫治疗提供潜在靶点。
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4. **文献名称**：*Monoclonal antibodies against recombinant calretinin: Production and characterization*
**作者**：Winsky, L. et al.
**摘要**：该技术性文献报道了CALB2单克隆抗体的开发及验证，证明其高特异性和在免疫印迹、免疫荧光中的应用价值，为后续研究提供可靠工具。
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以上文献涵盖CALB2的基础功能、疾病关联及抗体开发，可根据实际需求进一步查阅原文。
The CALB2 antibody targets calretinin, a 29 kDa calcium-binding protein encoded by the CALB2 gene, belonging to the EF-hand protein family. Primarily expressed in the central and peripheral nervous systems, calretinin regulates intracellular calcium signaling, modulating neuronal excitability, synaptic plasticity, and neuroprotection. It is widely used as a biomarker in neuroanatomy to identify specific neuron populations, such as GABAergic interneurons in the cerebral cortex and retina.
In clinical pathology, CALB2 antibodies are critical diagnostic tools. Calretinin expression is a hallmark of mesothelial cells, aiding in distinguishing malignant mesothelioma (calretinin-positive) from adenocarcinoma. It also serves as a marker for sex cord-stromal tumors (e.g., granulosa cell tumors) in ovarian and testicular cancers.
Antibodies against calretinin are validated for techniques like immunohistochemistry (IHC), Western blot, and immunofluorescence (IF). Specific clones (e.g., DAK-Calret 1) are commonly used, with reactivity across species including humans, mice, and rats. Challenges include potential cross-reactivity with other calcium-binding proteins (e.g., calbindin-D28k) and variable expression in non-target tissues. Nonetheless, CALB2 antibodies remain indispensable in both neuroscience research and cancer diagnostics, offering insights into cellular function and disease mechanisms.
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