| WB | 咨询技术 | Human,Monkey |
| IF | 咨询技术 | Human,Monkey |
| IHC | 咨询技术 | Human,Monkey |
| ICC | 技术咨询 | Human,Monkey |
| FCM | 咨询技术 | Human,Monkey |
| Elisa | 1/10000 | Human,Monkey |
| Aliases | EIF-5; EIF-5A |
| Entrez GeneID | 1983 |
| clone | 6D6G6 |
| WB Predicted band size | 49.2kDa |
| Host/Isotype | Mouse IgG2a |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Monkey |
| Immunogen | Purified recombinant fragment of human EIF5 (AA: 1-300) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于EIF5抗体的3篇模拟参考文献示例，基于学术文献的典型结构和内容概括：
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1. **文献名称**：*EIF5 Antibody Validation in Mammalian Translation Initiation Studies*
**作者**：Smith JL, et al.
**摘要**：本研究验证了EIF5抗体在检测真核翻译起始因子5(EIF5)表达的灵敏性和特异性。通过Western blot和免疫荧光实验，证实该抗体可特异性识别EIF5蛋白，并揭示其在调控mRNA翻译起始中的关键作用，为研究蛋白质合成机制提供可靠工具。
2. **文献名称**：*EIF5 Overexpression in Colorectal Cancer and Its Prognostic Significance*
**作者**：Wang Y, et al.
**摘要**：通过免疫组化(使用EIF5特异性抗体)分析结直肠癌组织样本，研究发现EIF5在肿瘤组织中显著高表达，且与患者不良预后相关。机制研究表明，EIF5通过激活AKT/mTOR通路促进肿瘤细胞增殖，提示其作为潜在治疗靶点。
3. **文献名称**：*Functional Interaction Between EIF5 and eIF2 in Translation Initiation*
**作者**：Kimura T, et al.
**摘要**：利用EIF5抗体进行免疫共沉淀实验，揭示了EIF5与eIF2复合物的动态相互作用，阐明其在翻译起始复合物组装中的调控机制。研究还发现EIF5的磷酸化状态影响其对核糖体结合的效率，为理解翻译失调相关疾病提供新视角。
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以上为模拟示例，实际文献需通过学术数据库(如PubMed、Web of Science)检索。如需具体文献，建议结合关键词“EIF5 antibody”、“EIF5 function”或“EIF5 cancer”进一步查询。
The eukaryotic initiation factor 5 (EIF5) is a critical component of the translation initiation machinery, playing a pivotal role in ribosomal subunit joining and start codon recognition during protein synthesis. As a GTPase-activating protein (GAP), EIF5 facilitates the hydrolysis of GTP bound to eIF2 within the 48S preinitiation complex, promoting the release of initiation factors and enabling the transition to elongation. Dysregulation of EIF5 has been implicated in cellular stress responses, oncogenesis, and metabolic disorders, making it a subject of interest in both basic and translational research.
EIF5 antibodies are essential tools for studying its expression, localization, and function in cellular processes. These antibodies are widely used in techniques such as Western blotting, immunofluorescence, and immunoprecipitation to investigate EIF5's role in translational control under physiological or pathological conditions. Specific EIF5 antibodies can distinguish between phosphorylated isoforms (e.g., EIF5-pS401), which are associated with stress granule formation during cellular stress. Researchers also utilize these antibodies to explore EIF5's interactions with other initiation factors (e.g., eIF2. eIF4B) and its potential as a biomarker in diseases like cancer, where aberrant translation initiation is frequently observed. Validation of antibody specificity remains crucial for accurate experimental interpretation.
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