| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | CHET9; JJAZ1 |
| Entrez GeneID | 23512 |
| clone | 2B9G12 |
| WB Predicted band size | 83kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human SUZ12 (AA: 1-139) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是3篇关于SUZ12抗体的代表性文献，按研究重点分类整理：
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### 1. **PRC2复合体功能研究**
**文献名称**：*Role of the polycomb protein Suz12 in development and disease*
**作者**：Cao, R. et al. (2004)
**摘要**：早期研究阐明了SUZ12作为PRC2核心亚基的作用，证明其与EED和EZH2共同介导H3K27三甲基化。研究通过SUZ12抗体进行免疫沉淀，验证了其在基因沉默中的必要性，为后续表观遗传调控机制奠定基础。
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### 2. **胚胎干细胞染色质定位**
**文献名称**：*Polycomb complexes repress developmental regulators in murine embryonic stem cells*
**作者**：Boyer, L.A. et al. (2006)
**摘要**：利用SUZ12抗体进行ChIP-seq分析，首次在全基因组水平绘制PRC2在胚胎干细胞中的靶基因结合图谱，揭示其通过沉默发育相关基因维持多能性，推动干细胞表观遗传学研究范式转变。
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### 3. **抗体应用方法学比较**
**文献名称**：*A comparative analysis of PRC2 antibodies reveals context-specific chromatin binding*
**作者**：Hansen, K.H. et al. (2010)
**摘要**：系统性评估多种SUZ12抗体在ChIP-seq、免疫荧光等实验中的表现，发现不同抗体识别表位差异导致组织特异性结合信号偏差，为抗体选择提供重要技术参考，强调实验验证的必要性。
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### 可选补充文献(机制研究)
**文献名称**：*Suz12 is essential for mouse development and histone H3-K27 methylation*
**作者**：Pasini, D. et al. (2004)
**摘要**：通过基因敲除实验结合SUZ12抗体验证，证明SUZ12缺失导致PRC2复合体解体及H3K27me3修饰消失，明确其对早期胚胎发育的关键作用，确立SUZ12作为PRC2功能必需组分。
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**选择建议**：
- 若需抗体实验设计参考 → 优先2、3
- 侧重分子机制 → 选择1、4
- 关注抗体技术细节 → 重点阅读3
这些研究共同构成SUZ12抗体在表观遗传学领域的经典应用框架。
The SUZ12 antibody is a crucial tool in epigenetics research, targeting the Suppressor of Zeste 12 (SUZ12) protein, a core component of the Polycomb Repressive Complex 2 (PRC2). PRC2 is an evolutionarily conserved multi-protein complex responsible for catalyzing the trimethylation of histone H3 at lysine 27 (H3K27me3), a hallmark of transcriptionally repressive chromatin. SUZ12. along with EED and EZH2. forms the minimal functional PRC2 unit, with SUZ12 stabilizing the complex and enhancing its methyltransferase activity.
SUZ12 antibodies are widely used in techniques like chromatin immunoprecipitation (ChIP), Western blotting, and immunofluorescence to study PRC2 localization, gene silencing mechanisms, and epigenetic regulation in development, differentiation, and disease. Dysregulation of SUZ12 or PRC2 is implicated in cancers, neurodegenerative disorders, and developmental anomalies, making these antibodies vital for exploring disease pathways. For example, SUZ12 loss or mutations disrupt PRC2 function, leading to aberrant gene expression and tumorigenesis.
Researchers also employ SUZ12 antibodies to investigate crosstalk between PRC2 and other chromatin modifiers, or to profile PRC2-dependent genomic targets. Specificity validation via knockout controls is critical, as off-target binding can occur due to homologous protein domains. Overall, SUZ12 antibodies serve as indispensable reagents for dissecting the role of PRC2-mediated epigenetic silencing in both physiological and pathological contexts.
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