| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GTF3C6 |
| Uniprot No | Q969F1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-213aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSMAAAADERSPEDGEDEEEEEQLVLVEL SGIIDSDFLSKCENKCKVLGIDTERPILQVDSCVFAGEYEDTLGTCVIFE ENVEHADTEGNNKTVLKYKCHTMKKLSMTRTLLTEKKEGEENIGGVEWLQ IKDNDFSYRPNMICNFLHENEDEEVVASAPDKSLELEEEEIQMNDSSNLS CEQEKPMHLEIEDSGPLIDIPSETEGSVFMETQMLP |
| 预测分子量 | 26 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于GTF3C6重组蛋白的3篇虚构参考文献示例，结构按您的要求整理：
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1. **文献名称**：*Cloning and Expression of Recombinant Human GTF3C6 in Escherichia coli*
**作者**：Smith J, et al.
**摘要**：本研究成功克隆了人类GTF3C6基因，并在大肠杆菌中实现重组表达。通过优化表达条件，获得了可溶性蛋白，为后续研究其与RNA聚合酶III的相互作用提供了基础材料。
2. **文献名称**：*Functional Analysis of GTF3C6 in tRNA Transcription via Recombinant Protein Reconstitution*
**作者**：Chen L, Wang H.
**摘要**：利用重组GTF3C6蛋白与转录因子复合体(TFIIIC)进行体外组装实验，证实GTF3C6对tRNA基因启动子的特异性识别具有调控作用，揭示了其在RNA聚合酶III转录中的分子机制。
3. **文献名称**：*Structural Characterization of Recombinant GTF3C6 Using Cryo-EM*
**作者**：Gomez R, et al.
**摘要**：通过冷冻电镜技术解析了重组GTF3C6蛋白的三维结构，发现其N端结构域在与DNA结合中的关键功能，为开发靶向转录失调疾病的策略提供了结构依据。
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注：以上文献为示例性内容，实际研究中请参考真实发表的论文。如需查找真实文献，建议在PubMed或Google Scholar中检索关键词“GTF3C6 recombinant protein”或“GTF3C6 gene expression”。
**Background of GTF3C6 Recombinant Protein**
GTF3C6 (General Transcription Factor 3C Subunit 6) is a protein-coding gene that encodes a subunit of the General Transcription Factor 3C (GTF3C) complex. This complex is essential for RNA polymerase III (Pol III)-mediated transcription, which is responsible for synthesizing small non-coding RNAs, including transfer RNAs (tRNAs), 5S ribosomal RNA (rRNA), and other regulatory RNAs. The GTF3C complex facilitates promoter recognition, assembly of the transcription preinitiation complex, and recruitment of Pol III to target genes. GTF3C6. specifically, contributes to the structural integrity and functional regulation of the complex, though its precise mechanistic role remains under investigation.
Recombinant GTF3C6 protein is produced through heterologous expression systems (e.g., *E. coli*, yeast, or mammalian cells*) using genetic engineering techniques. This allows large-scale production of the purified protein for functional and structural studies. Researchers utilize recombinant GTF3C6 to explore its interactions within the GTF3C complex, its role in Pol III-dependent transcription, and potential regulatory crosstalk with other cellular pathways, such as cell growth and proliferation.
Dysregulation of Pol III transcription is linked to diseases like cancer and neurological disorders, making GTF3C6 a subject of interest in understanding pathological mechanisms. Recombinant GTF3C6 also serves as a tool for developing assays to screen modulators of Pol III activity, with therapeutic implications. Studies often employ techniques like co-immunoprecipitation, chromatin immunoprecipitation (ChIP), and *in vitro* transcription assays to dissect its function.
The availability of recombinant GTF3C6 enables detailed biochemical characterization, including post-translational modifications and structure-function analyses, advancing insights into transcriptional regulation and disease biology. Its applications extend to diagnostics and drug discovery, particularly in targeting Pol III-driven pathologies.
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