| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GTF2H5 |
| Uniprot No | Q6ZYL4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-71aa |
| 氨基酸序列 | MVNVLKGVLIECDPAMKQFLLYLDESNALGKKFIIQDIDDTHVFVIAELVNVLQERVGELMDQNAFSLTQK |
| 预测分子量 | 35.1kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于GTF2H5(TTDA)重组蛋白研究的3篇代表性文献概览(文献名称及摘要内容为模拟，供参考)：
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1. **文献名称**：*"Structural and functional analysis of recombinant GTF2H5 (TTDA) in nucleotide excision repair"*
**作者**：Giglia-Mari et al.
**摘要**：研究通过重组表达纯化人源GTF2H5蛋白，解析其与TFIIH复合物的相互作用，揭示TTDA突变导致DNA修复缺陷的分子机制，为毛发低硫营养不良症(TTD)提供病理模型。
2. **文献名称**：*"Recombinant TTDA protein restores UV resistance in XPD-deficient cells"*
**作者**：Botta et al.
**摘要**：利用重组GTF2H5蛋白进行体外功能互补实验，证明其在恢复XPD突变细胞的紫外线损伤修复能力中的作用，提示潜在基因治疗策略。
3. **文献名称**：*"Expression and characterization of human GTF2H5 in E. coli: implications for TFIIH complex assembly"*
**作者**：Coin et al.
**摘要**：报道在大肠杆菌中高效表达可溶性重组GTF2H5蛋白，并验证其参与TFIIH复合物组装的关键结构域，为研究转录与修复机制提供工具。
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**实际文献检索建议**：
可通过PubMed(https://pubmed.ncbi.nlm.nih.gov/)搜索关键词 **"GTF2H5 recombinant"** 或 **"TTDA protein expression"** 获取最新研究。例如：
- 输入检索式：`("GTF2H5" OR "TTDA") AND ("recombinant protein" OR "protein expression")`
- 筛选与结构、功能或疾病模型相关的实验论文。
**Background of GTF2H5 Recombinant Protein**
GTF2H5 (General Transcription Factor IIH Subunit 5), also known as TTDA or p8. is a critical component of the transcription factor IIH (TFIIH) complex, which plays dual roles in transcription initiation and DNA repair. The TFIIH complex is essential for RNA polymerase II-mediated transcription, facilitating promoter unwinding, and for nucleotide excision repair (NER), a pathway that removes bulky DNA lesions caused by UV radiation or chemical agents. GTF2H5 stabilizes the interaction between the core TFIIH and the CAK (CDK-activating kinase) subcomplex, ensuring structural and functional integrity of the entire complex.
Mutations in the *GTF2H5* gene are linked to the autosomal recessive disorder trichothiodystrophy (TTD), characterized by brittle hair, developmental defects, and photosensitivity due to impaired NER. Recombinant GTF2H5 protein, produced through heterologous expression systems (e.g., *E. coli* or mammalian cells), retains biochemical activity and is widely used to study TFIIH assembly, transcription mechanisms, and DNA repair processes. Its recombinant form enables detailed analyses of protein-protein interactions, enzymatic assays, and structural studies (e.g., X-ray crystallography or cryo-EM) to elucidate how GTF2H5 dysfunction contributes to disease.
Additionally, recombinant GTF2H5 serves as a tool for screening therapeutic compounds targeting TFIIH-related disorders or enhancing DNA repair capacity in cancer therapies. Research on this protein continues to advance understanding of transcription-coupled repair mechanisms and their implications in genetic diseases and genomic stability.
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