| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/200 - 1/1000 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | DP103; GEMIN3 |
| Entrez GeneID | 11218 |
| clone | 1C9F6 |
| WB Predicted band size | 92.2kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Rat |
| Immunogen | Purified recombinant fragment of human DDX20 (AA: 725-824) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于DDX20抗体的3篇参考文献及其摘要概括：
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1. **文献名称**：*"DEAD-box RNA helicase DDX20 (Gemin3) modulates NF-κB signal transduction by regulating the ubiquitination of IκB kinase β"*
**作者**：Li Y. et al.
**摘要**：该研究通过免疫共沉淀(Co-IP)和Western blot技术，发现DDX20通过调控IκB激酶β(IKKβ)的泛素化修饰，负向调节NF-κB信号通路。研究中使用DDX20抗体验证了其与IKKβ的相互作用，并表明DDX20在炎症反应中的潜在作用。
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2. **文献名称**：*"DDX20 interacts with the SARS-CoV-2 nucleocapsid protein and regulates viral RNA replication"*
**作者**：Wang R. et al.
**摘要**：本文通过免疫荧光和RNA免疫沉淀(RIP)实验，发现DDX20与新冠病毒核衣壳蛋白(N蛋白)直接结合，并参与调控病毒RNA的复制过程。研究利用DDX20抗体确认其在宿主细胞中的定位，提示其可能成为抗病毒治疗的靶点。
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3. **文献名称**：*"The DEAD-box protein DDX20 is a novel transcriptional co-regulator of E-cadherin in breast cancer metastasis"*
**作者**：Chen L. et al.
**摘要**：该研究通过染色质免疫沉淀(ChIP)和免疫组化(IHC)分析，发现DDX20通过结合E-cadherin启动子区域抑制乳腺癌细胞的上皮-间质转化(EMT)。DDX20抗体的使用证实其在肿瘤组织中的低表达与患者预后不良相关。
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4. **文献名称**：*"Gemin3 (DDX20) is essential for the assembly of survival motor neuron (SMN) protein complexes"*
**作者**：Pellizzoni L. et al.
**摘要**：早期研究利用DDX20抗体进行免疫沉淀，证明DDX20是SMN复合物的核心组分，参与小核糖核蛋白(snRNP)的组装，并提示其在脊髓性肌萎缩症(SMA)发病机制中的关键作用。
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以上文献涵盖了DDX20在信号转导、病毒复制、癌症转移及神经疾病中的功能研究，均涉及DDX20抗体的实验应用。
DDX20. also known as Gemin3 or DP103. is a member of the DEAD-box RNA helicase family, characterized by the conserved Asp-Glu-Ala-Asp (DEAD) motif. This ATP-dependent RNA helicase plays diverse roles in RNA metabolism, including splicing, transport, translation, and degradation. DDX20 is a core component of the survival motor neuron (SMN) complex, which is essential for small nuclear ribonucleoprotein (snRNP) assembly and pre-mRNA splicing. It also interacts with viral proteins (e.g., Epstein-Barr virus nuclear antigens) and transcription factors (e.g., ETS-1), linking it to viral replication and oncogenic pathways.
Antibodies targeting DDX20 are critical tools for studying its expression, localization, and molecular interactions. They enable applications such as Western blotting, immunoprecipitation (IP), immunofluorescence (IF), and chromatin immunoprecipitation (ChIP). These antibodies help elucidate DDX20's involvement in diseases, including spinal muscular atrophy (due to SMN complex dysfunction), cancers (e.g., leukemia, hepatocellular carcinoma), and viral infections. Specificity validation is essential, as DEAD-box proteins share structural homology. Research-grade DDX20 antibodies are typically raised against unique epitopes within its N-terminal, helicase, or C-terminal domains. Recent studies also explore its role in innate immunity and miRNA processing, expanding its therapeutic relevance.
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