| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/200 - 1/1000 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | DP103; GEMIN3 |
| Entrez GeneID | 11218 |
| clone | 7B2B1 |
| WB Predicted band size | 92.2kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human DDX20 (AA: 725-824) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是3篇关于DDX20抗体的参考文献摘要(文献标题与作者为虚拟示例，仅供格式参考)：
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1. **标题**：DDX20 regulates antiviral innate immunity through RIG-I signaling
**作者**：Chen J et al.
**摘要**：研究揭示DDX20通过调控RIG-I介导的I型干扰素信号通路参与抗病毒免疫反应，Western blot及免疫共沉淀实验中使用DDX20抗体验证其与MAVS蛋白的相互作用。
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2. **标题**：DDX20 interacts with non-coding RNAs to modulate chromatin remodeling
**作者**：Wang L et al.
**摘要**：本文发现DDX20通过结合特定lncRNA调控染色质重塑复合体，免疫荧光实验采用DDX20抗体定位其细胞核内分布，提示其在表观遗传调控中的新功能。
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3. **标题**：DDX20 as a biomarker in hepatocellular carcinoma progression
**作者**：Kimura T et al.
**摘要**：通过免疫组化分析肝癌组织，DDX20抗体显示其在肿瘤组织中高表达，且与患者预后不良相关，提示其作为潜在治疗靶点的价值。
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注：以上文献为模拟示例，实际研究中建议通过PubMed等数据库以“DDX20”、“Gemin3”、“DEAD-box protein 20”为关键词检索最新文献。
DDX20. also known as Gemin3 or DP103. is a member of the DEAD-box family of RNA helicases, which play essential roles in RNA metabolism, including transcription, splicing, and translation. This ATP-dependent enzyme is a core component of the survival motor neuron (SMN) complex, involved in spliceosomal small nuclear ribonucleoprotein (snRNP) assembly and pre-mRNA processing. DDX20 also interacts with viral proteins, such as HIV-1 Rev, and participates in innate immunity by regulating type I interferon signaling.
Antibodies targeting DDX20 are critical tools for studying its function in RNA biology, viral pathogenesis, and disease mechanisms. They are widely used in techniques like Western blotting, immunoprecipitation, immunofluorescence, and chromatin immunoprecipitation (ChIP) to detect DDX20 expression, localization, and protein interactions. Dysregulation of DDX20 has been implicated in cancers (e.g., breast cancer, hepatocellular carcinoma) and neurological disorders like spinal muscular atrophy (SMA), where SMN complex dysfunction occurs. Researchers also explore its role in autoimmune diseases and viral infections, including HIV and hepatitis C.
Commercially available DDX20 antibodies are typically raised in rabbits or mice, with validation across multiple applications and species (human, mouse, rat). Specificity is confirmed via knockout controls or siRNA knockdown. These reagents support investigations into DDX20’s dual roles as a helicase and a transcriptional co-regulator, particularly its interaction with transcription factors like ETS-1 or MYC in oncogenesis.
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