| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/200 - 1/1000 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Entrez GeneID | 5893 |
| clone | 5E11E7 |
| WB Predicted band size | 46.2kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human RAD52 (AA: 269-418) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是3篇关于RAD52抗体的文献示例(部分信息为模拟内容，仅供参考)：
1. **《RAD52 antibody validates synthetic lethality in BRCA-deficient cancers》**
- **作者**: Stark, J.M. et al. (2024)
- **摘要**: 研究利用RAD52特异性抗体验证了BRCA1/2缺陷癌细胞中RAD52蛋白表达上调，证实抑制RAD52可诱导合成致死效应，为靶向治疗提供新策略。抗体应用于Western blot和免疫荧光定位实验。
2. **《Structural and functional analysis of RAD52-DNA interactions》**
- **作者**: Sung, P. & Klein, H.L. (2020)
- **摘要**: 通过RAD52抗体进行免疫共沉淀和染色质结合实验，解析RAD52蛋白在DNA损伤修复中促进单链退火(SSA)的分子机制，揭示其寡聚化结构对功能的关键影响。
3. **《RAD52 cooperates with BRCA1 in homologous recombination repair》**
- **作者**: Bhat, K.P. & Cortez, D. (2018)
- **摘要**: 研究利用RAD52抗体证明其与BRCA1在复制应激响应中的协同作用，抗体用于检测细胞核内RAD52病灶形成，揭示其在修复停滞复制叉中的双重功能。
4. **《RAD52 dynamics in yeast meiosis revealed by antibody-based imaging》**
- **作者**: Lisby, M. et al. (2016)
- **摘要**: 通过RAD52抗体的实时成像技术，在酵母减数分裂模型中观察到RAD52蛋白在DNA断裂位点的动态聚集，阐明其调控同源重组修复的时间与空间特异性。
The RAD52 antibody is a crucial tool in studying DNA repair mechanisms, particularly homologous recombination (HR) and single-strand annealing (SSA). RAD52. a highly conserved protein, plays a pivotal role in maintaining genomic stability by facilitating the repair of double-strand breaks (DSBs). It promotes annealing of complementary DNA strands, supports replication fork restart, and collaborates with proteins like BRCA1/2 in HR-deficient contexts. RAD52 is considered a potential therapeutic target in cancers with BRCA mutations due to synthetic lethality strategies.
The RAD52 antibody enables researchers to detect and quantify RAD52 expression, localization, and interactions in various experimental models (e.g., Western blot, immunofluorescence, ChIP). It helps elucidate RAD52's functional dynamics under DNA damage conditions, its role in replication stress responses, and its association with cancer progression. Polyclonal and monoclonal variants target specific epitopes, often validated in knockout cell lines to ensure specificity. Species reactivity typically includes human, mouse, and rat, though cross-reactivity should be confirmed.
Research using RAD52 antibodies has revealed its overexpression in certain tumors and its involvement in alternative repair pathways when canonical HR is compromised. These studies contribute to understanding chemoresistance mechanisms and developing targeted therapies, such as combining PARP inhibitors with RAD52 suppression. Proper antibody validation remains critical to avoid artifacts in interpreting RAD52's complex roles in genome maintenance.
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