Mouse Monoclonal SIRT4 Antibody

SIRT4 antibodies are essential tools for studying the role of SIRT4. a member of the sirtuin family of NAD⁺-dependent deacylases. SIRT4 is primarily localized in mitochondria and plays a critical role in regulating metabolic pathways, including insulin secretion, fatty acid oxidation, and amino acid metabolism. Unlike other sirtuins (e.g., SIRT1 or SIRT3), SIRT4 exhibits weak deacetylase activity but is involved in ADP-ribosylation and lipoamidase functions, influencing energy homeostasis and stress responses. Researchers use SIRT4 antibodies in techniques like Western blotting, immunohistochemistry, and immunofluorescence to detect SIRT4 expression, subcellular localization, and post-translational modifications across tissues. These antibodies are particularly valuable in studying metabolic disorders, cancer, and aging-related diseases, as SIRT4 dysregulation has been linked to tumor suppression, mitochondrial dysfunction, and metabolic syndrome. Validating antibody specificity is crucial, as cross-reactivity with other sirtuin isoforms (e.g., SIRT3 or SIRT5) can lead to inaccurate interpretations. Recent studies also explore SIRT4's potential as a therapeutic target or biomarker, driving demand for high-quality, well-characterized antibodies. Commercial SIRT4 antibodies are typically raised against conserved peptide regions, with validation in knockout models to ensure reliability.