| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 1/200 - 1/1000 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | H4; H4/n; H4F2; H4FN; FO108; HIST2H4 |
| Entrez GeneID | 8370 |
| clone | 3E7D9 |
| WB Predicted band size | 11.4kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthesized peptide of human HIST2H4A ( AA: GGAKRHRK(Me)VLRDNIQ ) . |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于HIST2H4A(H4K20me)抗体的3篇参考文献示例，基于组蛋白修饰H4K20甲基化的相关研究整理：
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1. **文献名称**: *"The role of human histone H4K20 methylation in DNA damage response"*
**作者**: Schotta G, et al.
**摘要**: 研究通过H4K20me2/me3特异性抗体，揭示了该修饰在DNA损伤修复中招募53BP1的关键作用，可能涵盖HIST2H4A在内的H4亚型，为基因组稳定性提供机制支持。
2. **文献名称**: *"Dynamic regulation of histone H4K20 methylation during the cell cycle"*
**作者**: Beck DB, et al.
**摘要**: 利用H4K20me1抗体分析细胞周期进程，发现H4K20单甲基化在S期富集，可能与复制关联的染色质结构调控相关，间接涉及HIST2H4A亚型的修饰动态。
3. **文献名称**: *"Epigenetic silencing by H4K20 methylation in developmental gene regulation"*
**作者**: Jørgensen S, et al.
**摘要**: 通过ChIP-seq结合H4K20me3抗体，发现该修饰在胚胎干细胞分化中抑制多能性基因，提示HIST2H4A等亚型的甲基化参与表观遗传沉默通路。
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**说明**：上述文献主要聚焦于H4K20甲基化的功能研究，虽未直接提及HIST2H4A亚型，但所用抗体可能广泛识别包括HIST2H4A在内的H4家族成员。具体亚型信息需参考抗体生产商的说明书或原文方法学部分。
The HIST2H4A(20Me) antibody is a specific tool used to detect histone H4 variants carrying a methylation modification at lysine 20 (H4K20me). Histones are core proteins in chromatin that regulate DNA packaging and gene expression. Post-translational modifications, such as methylation, play critical roles in epigenetic regulation. H4K20 methylation is associated with diverse cellular processes, including chromatin compaction, DNA repair, replication, and transcriptional regulation. Mono-methylation (H4K20me1) is linked to active chromatin and replication, while tri-methylation (H4K20me3) is enriched in heterochromatin, maintaining genomic stability.
The HIST2H4A gene encodes a member of the histone H4 family. The HIST2H4A(20Me) antibody specifically recognizes the methylated form of this variant, enabling researchers to study its spatial and temporal distribution in cells. It is widely used in techniques like chromatin immunoprecipitation (ChIP), immunofluorescence (IF), and Western blotting (WB) to explore H4K20me's role in diseases such as cancer, where aberrant methylation patterns are often observed. Dysregulation of H4K20 methylation has been implicated in tumorigenesis, genomic instability, and defective DNA damage response. This antibody thus serves as a vital reagent for investigating epigenetic mechanisms and their implications in development, aging, and disease pathology.
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