| WB | 1/500 - 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | MEK5; MAPKK5; PRKMK5; HsT17454 |
| Entrez GeneID | 5607 |
| clone | 5H11B10 |
| WB Predicted band size | 50kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human MAP2K5 (AA: 63-180) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3条关于MAP2K5抗体的模拟参考文献示例(注：文献为虚构示例，实际文献请通过PubMed或Google Scholar查询)：
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1. **文献名称**: "Development and Validation of a Specific MAP2K5 Antibody for Detecting Phosphorylated Isoforms in Human Tissues"
**作者**: Chen L, et al.
**摘要**: 该研究开发了一种高特异性MAP2K5抗体，可识别磷酸化与非磷酸化形式的蛋白。通过Western blot和免疫组化验证其在肺癌和正常组织中的差异表达，并证明其与ERK5信号通路激活的相关性。
2. **文献名称**: "MAP2K5 Antibody Application in Cardiac Hypertrophy Models: Implications for Stress Response Signaling"
**作者**: Tanaka K, et al.
**摘要**: 使用MAP2K5抗体探究心肌肥大模型中ERK5通路的调控机制，发现MAP2K5在压力超负荷下的表达上调，并与其下游转录因子MEF2C的活性变化相关，为心血管疾病治疗提供新靶点。
3. **文献名称**: "Cross-Reactivity Analysis of Commercial MAP2K5 Antibodies: Implications for Neurodegenerative Disease Research"
**作者**: Gupta R, et al.
**摘要**: 系统性评估了市售MAP2K5抗体的特异性，发现部分抗体存在与MAP2K1等旁系同源蛋白的交叉反应，强调抗体验证在阿尔茨海默病模型研究中的必要性，并推荐了优化实验方案。
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如需真实文献，建议检索关键词：**MAP2K5 antibody**, **MEK5 antibody**, 或结合研究领域如 **MAP2K5 cancer**/**neurodegeneration**。
The MAP2K5 antibody targets mitogen-activated protein kinase kinase 5 (MAP2K5 or MEK5), a serine/threonine kinase within the MAPK signaling pathway. MAP2K5 acts as an upstream activator of ERK5 (extracellular signal-regulated kinase 5), regulating cellular responses to stress, growth factors, and cytokines. This pathway influences cell proliferation, differentiation, survival, and apoptosis, with ERK5 uniquely involved in both nuclear signaling and cytoskeletal reorganization. MAP2K5 antibodies are widely used in research to study ERK5 pathway dynamics, particularly in cancer, cardiovascular diseases, and neurodegenerative disorders.
These antibodies are essential tools for detecting MAP2K5 expression via techniques like Western blotting, immunohistochemistry, and immunofluorescence. Specificity is validated through knockout controls or siRNA knockdown to ensure minimal cross-reactivity with other MAPK family members. MAP2K5/ERK5 signaling has dual roles in cancer—acting as a tumor suppressor in some contexts or promoting metastasis in others—making its study critical for therapeutic targeting. Additionally, the pathway’s involvement in endothelial cell function and neuronal survival highlights its broader physiological relevance.
Commercial MAP2K5 antibodies are typically raised in rabbits or mice, with recommended applications and dilution ratios provided by manufacturers. Researchers rely on these reagents to explore disease mechanisms, validate gene-editing models, or assess drug efficacy targeting the ERK5 cascade.
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