| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/200 - 1/1000 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | MCS; MCSP; HSMCSGEN1 |
| Entrez GeneID | 4184 |
| clone | 5C10D8 |
| WB Predicted band size | 12.8kDa |
| Host/Isotype | Mouse IgG2b |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human SMCP (AA: FULL(1-116)) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
+ +
以下为假设性示例文献(仅供格式参考，具体内容需通过学术数据库验证)：
---
1. **文献名称**: "Developmental expression and functional analysis of SMCP in mammalian spermatogenesis"
**作者**: Mori C, et al.
**摘要**: 本研究通过制备高特异性SMCP多克隆抗体，系统检测了小鼠精子发生过程中线粒体鞘的形态变化，证实SMCP蛋白对线粒体结构的稳定性和精子运动能力具有关键作用。
---
2. **文献名称**: "Cross-species reactivity evaluation of anti-SMCP antibodies in vertebrate models"
**作者**: Naemi F, et al.
**摘要**: 评估了SMCP抗体在人类、小鼠及斑马鱼中的交叉反应性，发现抗体在哺乳动物中特异性较强，为跨物种生殖生物学研究提供了实验工具。
---
3. **文献名称**: "SMCP antibody-based diagnostic potential in male infertility screening"
**作者**: Zhang Y, et al.
**摘要**: 利用SMCP单克隆抗体开发了精子线粒体功能缺陷的免疫荧光检测方法，临床数据显示其与弱精症存在显著相关性，提示诊断应用价值。
---
4. **文献名称**: "Structural insights into SMCP-antibody interactions by cryo-EM"
**作者**: Kwon J, et al.
**摘要**: 通过冷冻电镜解析SMCP与其单克隆抗体的复合物结构，揭示了抗体结合表位与线粒体鞘组装的关键功能域重叠，为靶向药物设计提供依据。
---
**建议**：可通过PubMed、Google Scholar等平台以关键词"SMCP antibody"+"sperm mitochondria"或"male fertility"检索真实文献。部分经典研究集中在2000-2015年间，近年则侧重结构解析与临床转化。
Sperm Mitochondria-associated Cysteine-rich Protein (SMCP) is a testis-specific protein predominantly expressed during spermatogenesis. It localizes to the mitochondrial sheath of sperm flagella, playing a critical role in stabilizing mitochondrial organization and ensuring proper sperm motility. Structurally, SMCP is characterized by a high cysteine content, facilitating disulfide bond formation and structural resilience essential for sperm function.
SMCP antibodies are valuable tools in reproductive biology research, particularly for studying sperm development, mitochondrial dynamics, and male infertility mechanisms. They enable the detection and localization of SMCP in spermatozoa, aiding investigations into its role in energy metabolism and structural integrity during fertilization. Dysregulation or mutations in SMCP are linked to asthenozoospermia (reduced sperm motility) and subfertility, making these antibodies relevant in clinical diagnostics and research on assisted reproductive technologies.
Additionally, SMCP antibodies may help identify autoimmune infertility cases where anti-sperm antibodies target SMCP, disrupting mitochondrial function. Their application extends to animal studies, such as transgenic models evaluating SMCP's role in fertility. Overall, SMCP antibodies bridge basic research and clinical insights, advancing understanding of sperm biology and therapeutic strategies for male reproductive disorders.
×
