| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 1/200 - 1/1000 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Entrez GeneID | 5617 |
| clone | 2H10H5 |
| WB Predicted band size | 26kDa |
| Host/Isotype | Mouse IgG2b |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human PRL (AA: 65-173) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于PRL(催乳素)抗体的3篇文献示例及其摘要概括(注：文献信息为模拟生成，仅供参考，实际引用需核实原文)：
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1. **文献名称**：*Development of a High-Specificity Monoclonal Antibody for Human Prolactin Detection*
**作者**：Smith J, et al.
**摘要**：本研究报道了一种新型PRL单克隆抗体的开发与验证。该抗体通过杂交瘤技术制备，在ELISA和Western blot中表现出高灵敏度和特异性，成功应用于血清样本中PRL的定量检测，为临床诊断垂体功能异常提供了可靠工具。
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2. **文献名称**：*Comparative Analysis of PRL Antibodies in Immunohistochemical Staining of Pituitary Adenomas*
**作者**：Zhang L, et al.
**摘要**：文章比较了五种商业PRL抗体在垂体腺瘤组织中的免疫组化表现。结果显示，克隆号“3D4”的抗体在染色强度和特异性上最优，且与患者血清PRL水平显著相关，提示其在病理诊断中的潜在应用价值。
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3. **文献名称**：*Role of PRL in Breast Cancer: Insights from Antibody-Mediated Receptor Blockade*
**作者**：Johnson R, et al.
**摘要**：通过使用PRL受体中和抗体，本研究探讨了PRL信号通路在乳腺癌细胞增殖中的作用。实验表明，抗体阻断PRL受体可显著抑制肿瘤细胞的迁移和侵袭，提示靶向PRL通路可能成为乳腺癌治疗的新策略。
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如需实际文献，建议通过PubMed、Google Scholar等平台检索关键词(如“prolactin antibody”、“PRL immunoassay”)，并筛选近年高被引或权威期刊论文。
**Background of PRL Antibodies**
Prolactin (PRL), a peptide hormone primarily synthesized by the anterior pituitary gland, plays critical roles in lactation, reproduction, and immune regulation. PRL antibodies, including monoclonal and polyclonal variants, are essential tools for detecting and quantifying PRL in research and clinical diagnostics. These antibodies target specific epitopes on the PRL molecule, enabling applications such as Western blotting, immunohistochemistry (IHC), and enzyme-linked immunosorbent assays (ELISA).
Elevated PRL levels (hyperprolactinemia) are associated with conditions like prolactinomas, infertility, and autoimmune disorders. PRL antibodies aid in diagnosing these pathologies by measuring serum or tissue PRL concentrations. Additionally, they are used to study PRL's extrapituitary sources (e.g., immune cells, mammary glands) and its role in pathophysiology, including autoimmune diseases (e.g., lupus, rheumatoid arthritis) where PRL may act as a cytokine.
Autoantibodies against PRL, though rare, have been reported in autoimmune hypophysitis and prolactinomas, complicating hormone measurement. Thus, PRL antibody assays require validation to avoid cross-reactivity with homologous hormones (e.g., growth hormone) or binding interference from autoantibodies.
Overall, PRL antibodies are indispensable for advancing endocrine research, improving diagnostic accuracy, and exploring therapeutic interventions targeting PRL-related disorders.
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