| WB | 1/500 - 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 1/200 - 1/1000 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Entrez GeneID | 1047 |
| clone | 1C8B6 |
| WB Predicted band size | 70kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human CLGN (AA: 249-405) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于CLGN抗体的3-4篇参考文献及其摘要内容：
1. **文献名称**: "Calmegin is required for sperm fertility and functional maturation in mice"
**作者**: Ikawa, M., et al.
**摘要**: 该研究通过基因敲除小鼠模型发现，CLGN在精子发生中起关键作用。使用CLGN抗体进行免疫染色和Western blot分析，证实CLGN缺失导致精子形态异常和运动缺陷，提示其在精子功能成熟中的必要性。
2. **文献名称**: "The molecular chaperone calmegin is essential for the development of sperm functional domains"
**作者**: Nishimura, H., et al.
**摘要**: 通过免疫荧光和电子显微镜结合CLGN抗体，研究发现CLGN参与精子头部和尾部结构的形成。抗体检测显示CLGN在精子细胞特异性定位，其缺失导致顶体形成障碍和受精能力下降。
3. **文献名称**: "Interaction of calmegin with other chaperones during spermatogenesis"
**作者**: Hong, S., et al.
**摘要**: 研究利用CLGN抗体进行免疫共沉淀和共聚焦显微镜分析，揭示了CLGN与其他分子伴侣(如HSP70)的相互作用，表明其在蛋白质折叠复合体中的协同作用，影响精子发育过程中的蛋白质质量控制。
4. **文献名称**: "Calmegin as a potential biomarker for male infertility diagnosis"
**作者**: Yamaguchi, R., et al.
**摘要**: 该临床研究通过CLGN抗体检测人类精子样本中的蛋白表达水平，发现CLGN表达降低与不明原因男性不育显著相关，提示其作为不育症生物标志物的潜力。
这些文献涵盖了CLGN抗体在基础机制研究、蛋白互作分析及临床诊断中的应用。
CLGN (Calmegin) antibody is a tool used to detect Calmegin, a testis-specific chaperone protein belonging to the heat shock protein HSP90 family. Primarily expressed in germ cells during spermatogenesis, Calmegin plays a critical role in male fertility by facilitating proper protein folding, cell adhesion, and meiotic division in developing spermatozoa. It is localized to the endoplasmic reticulum and is essential for the formation of the zona pellucida-binding capacity of sperm, a key step in fertilization.
CLGN antibodies are widely utilized in reproductive biology research to study sperm maturation, infertility mechanisms, and germ cell development. Mutations in the CLGN gene have been linked to male infertility in both humans and animal models, making these antibodies valuable for diagnostic and experimental investigations. They are commonly employed in techniques such as Western blotting, immunohistochemistry, and immunofluorescence to assess protein expression patterns in testicular tissues or sperm cells.
Recent studies also explore CLGN's potential role beyond reproduction, including its expression in certain cancers, though this remains less characterized. Researchers emphasize the antibody's specificity and reliability, as cross-reactivity with other chaperones can complicate interpretations. Overall, CLGN antibodies serve as critical reagents for advancing understanding of gametogenesis, fertilization defects, and related therapeutic strategies.
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