| WB | 1/500 - 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | STSL |
| Entrez GeneID | 64240 |
| clone | 1B5E10 |
| WB Predicted band size | 72.5kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human ABCG5 (AA: 306-367) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于ABCG5抗体的3篇参考文献概览，包含文献名称、作者及摘要内容概括：
1. **《ABCG5/ABCG8 in cholesterol excretion and atherosclerosis》**
- 作者：Yu L, von Bergmann K, Lutjohann D, et al.
- 摘要：研究利用ABCG5抗体分析ABCG5/ABCG8蛋白在肝脏和肠道中的表达，探讨其在胆固醇排泄中的作用，并发现其功能缺陷可能导致动脉粥样硬化风险增加。
2. **《Immunolocalization of ABCG5 and ABCG8 in human liver and gallstones》**
- 作者：Kosters A, Jirsa M, Groen AK, et al.
- 摘要：通过免疫组化结合ABCG5特异性抗体，揭示ABCG5在肝细胞胆小管膜的高表达，并发现其在胆囊结石患者中的表达异常，提示其与胆汁胆固醇过饱和相关。
3. **《Role of ABCG5 mutations in sitosterolemia pathogenesis》**
- 作者：Berge KE, Tian H, Graf GA, et al.
- 摘要：使用ABCG5抗体检测患者组织样本，证实ABCG5基因突变导致蛋白功能丧失，引发植物甾醇蓄积症(sitosterolemia)，为疾病诊断提供分子依据。
ABCG5 antibodies are essential tools for studying the ATP-binding cassette (ABC) transporter subfamily G member 5. a protein critical for sterol homeostasis. ABCG5 partners with ABCG8 to form a heterodimeric transporter expressed predominantly in the liver and intestine. This complex facilitates the efflux of dietary cholesterol and plant sterols into bile and the intestinal lumen, preventing their excessive absorption. Mutations in the ABCG5 gene are linked to sitosterolemia, a rare genetic disorder characterized by hyperabsorption and reduced excretion of sterols, leading to xanthomas and premature cardiovascular disease.
ABCG5 antibodies are widely used in research to detect protein expression, localization, and function via techniques like Western blotting, immunohistochemistry, and immunofluorescence. They help elucidate the transporter's role in lipid metabolism, drug resistance, and disease mechanisms. Commercially available antibodies are typically raised against specific epitopes, such as the N-terminal or cytoplasmic domains, and validated for specificity in human or murine tissues. Recent studies also explore ABCG5's potential in cancer biology, as its overexpression in certain tumors may influence chemotherapeutic responses. Understanding ABCG5 dynamics through antibody-based assays remains pivotal for developing therapeutic strategies targeting sterol-related disorders.
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