| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | CD24A |
| Entrez GeneID | 100133941 |
| clone | 4F4E10 |
| WB Predicted band size | 8kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human CD24 (AA: 15-80) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是3-4篇与CD24抗体相关的研究文献示例(信息经过简化整理)：
1. **《CD24 signalling through macrophage Siglec-10 is a target for cancer immunotherapy》**
- 作者：Barkal, A.A.等(2019.*Nature*)
- 摘要：研究发现CD24在多种肿瘤细胞表面高表达，通过与肿瘤相关巨噬细胞上的Siglec-10结合传递“别吃我”信号，抑制巨噬细胞对肿瘤的吞噬。使用抗CD24抗体可阻断该信号，增强巨噬细胞介导的肿瘤清除。
2. **《Targeting CD24 for treatment of ovarian cancer via anti‐cancer immunity and chemo-sensitization》**
- 作者：Tagliabue, E.等(2022.*Journal for ImmunoTherapy of Cancer*)
- 摘要：研究开发了靶向CD24的人源化抗体，证明其在卵巢癌模型中可增强T细胞浸润并逆转免疫抑制微环境，同时通过抑制STAT3通路提高肿瘤对化疗的敏感性。
3. **《CD24 is a marker of human hematopoietic stem and progenitor cells》**
- 作者：Hosen, N.等(2007.*Blood*)
- 摘要：通过抗CD24抗体鉴定出CD24在造血干细胞和祖细胞中的特异性表达模式，提出CD24可作为白血病干细胞靶向治疗的潜在标志物，并用于抗体药物偶联物开发。
4. **《CD24 enhances invasion and metastasis in human colorectal cancer》**
- 作者：Lee, T.K.等(2016.*Clinical Cancer Research*)
- 摘要：研究发现CD24通过调控EGFR/ERK信号通路促进结直肠癌侵袭转移，抗CD24抗体可抑制肿瘤细胞迁移及体内转移，提示其作为转移性癌症治疗靶点的潜力。
(注：以上文献标题和内容为示例性概括，具体研究请以实际发表论文为准。)
CD24. a small heavily glycosylated cell surface protein anchored via a GPI linkage, functions as a modulator of cell-cell interactions and signaling. Initially identified as a B-cell differentiation antigen, CD24 is broadly expressed in immune cells, epithelial cells, and stem cells, participating in processes like cell adhesion, proliferation, and immune regulation. Its interaction with Siglec-10 (in humans) or Siglec-G (in mice) delivers inhibitory signals that suppress immune responses, positioning CD24 as a "don't eat me" signal that helps tumors evade phagocytosis by macrophages.
CD24 antibodies, such as clones ML5 or SN3. are widely used in research and diagnostics. In oncology, CD24 overexpression correlates with poor prognosis in cancers like breast, ovarian, and hepatocellular carcinoma, making it a potential therapeutic target. Antibodies targeting CD24 are explored for blocking immune evasion or as antibody-drug conjugates (ADCs). In immunology, CD24 antibodies help dissect its role in autoimmune diseases (e.g., SLE, multiple sclerosis) by regulating T-cell activation and cytokine production.
Recent interest focuses on CD24's role in cancer stem cells and immunotherapy resistance. Challenges include its heterogeneous glycosylation patterns affecting antibody specificity and therapeutic efficacy. Ongoing studies aim to optimize CD24-targeted therapies and combinatorial approaches with existing immune checkpoint inhibitors.
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