| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 1/100 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | APH-1; APH-1A; CGI-78; 6530402N02Rik |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human APH1A |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于PIK3R1抗体的3篇参考文献示例(内容为虚构，仅作格式参考)：
1. **"PIK3R1 antibody-based detection of p85α expression in breast cancer progression"**
*Authors: Smith J, Lee R, et al.*
**摘要**: 研究使用特异性PIK3R1抗体通过免疫组化分析乳腺癌组织中p85α蛋白的表达，发现其低表达与肿瘤转移和不良预后显著相关，提示其在PI3K/AKT通路调控中的潜在作用。
2. **"Development of a monoclonal anti-PIK3R1 antibody for functional studies in metabolic disorders"**
*Authors: Chen L, Wang Y, et al.*
**摘要**: 报道一种新型高亲和力抗PIK3R1单克隆抗体的开发与验证，成功用于小鼠肝脏组织的Western blot和免疫沉淀，揭示PIK3R1在胰岛素信号传导及代谢异常中的调控机制。
3. **"Autoantibodies against PIK3R1 in autoimmune encephalitis: A case-control study"**
*Authors: Gonzalez S, Müller T, et al.*
**摘要**: 通过ELISA和蛋白质印迹法检测自身免疫性脑炎患者血清中的PIK3R1抗体，发现其阳性率显著高于健康对照组，提示该抗体可能作为新型生物标志物用于疾病诊断。
(注：以上文献为示例，实际文献需通过学术数据库检索获取。)
The PIK3R1 gene encodes the regulatory subunit p85α of phosphatidylinositol 3-kinase (PI3K), a critical signaling enzyme involved in cell growth, survival, metabolism, and apoptosis. As a regulatory component, p85α stabilizes the catalytic subunit (p110) of PI3K and modulates its lipid kinase activity. PIK3R1 antibodies are essential tools for studying the expression, localization, and functional interactions of this protein in physiological and pathological contexts. These antibodies are widely used in techniques such as Western blotting, immunohistochemistry (IHC), and immunoprecipitation (IP) to assess protein levels, tissue distribution, and molecular pathways in cancer, metabolic disorders, and immune diseases.
Mutations in PIK3R1 are linked to diseases including primary immunodeficiency, insulin resistance, and cancers. Researchers employ PIK3R1 antibodies to investigate how dysregulated PI3K signaling contributes to tumorigenesis, therapy resistance, or metabolic dysfunction. For example, altered p85α expression or activity has been observed in breast, ovarian, and colorectal cancers, making these antibodies valuable for mechanistic studies and biomarker validation. Commercially available PIK3R1 antibodies vary in specificity, with validation often performed using knockout cell lines or tissues to confirm target recognition. Challenges include cross-reactivity with other PI3K regulatory isoforms (e.g., p85β) and distinguishing post-translational modifications. Despite these limitations, PIK3R1 antibodies remain indispensable for unraveling the complex roles of PI3K signaling in health and disease.
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