Mouse Monoclonal POU3F2 Antibody

The POU3F2 antibody is a valuable tool for detecting the POU3F2 protein, a member of the POU-domain transcription factor family. POU3F2. also known as BRN2 or N-Oct-3. plays critical roles in neural development, cell differentiation, and oncogenesis. It regulates gene expression by binding to specific DNA sequences via its conserved POU domain, which consists of two DNA-binding subdomains (POU-specific and POU-homeodomain). In neuroscience, POU3F2 is essential for the maturation of hypothalamic neurons, pituitary gland development, and sensory cell differentiation. Notably, it has gained attention in cancer research due to its oncogenic role in melanoma, where it promotes tumor invasiveness, metastasis, and resistance to therapy by modulating MITF and other downstream targets. The POU3F2 antibody is widely used in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to study its expression, localization, and function in tissues or cultured cells. Researchers often validate its specificity using knockout controls or siRNA-mediated silencing. Commercial antibodies typically target epitopes within the N-terminal or POU domain, with cross-reactivity verified across human, mouse, and rat samples. Its application extends to both basic research exploring neural pathways and clinical studies investigating cancer biomarkers or therapeutic targets.