| WB | 1/500 - 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | GluA2; GluR2; gluR-B; GluR-K2 |
| Entrez GeneID | 2891 |
| clone | 7G6 |
| WB Predicted band size | 98.8kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human GRIA2 (AA: 652-807) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于GRIA2抗体的3篇模拟参考文献(虚构内容，仅供示例参考)：
1. **"GRIA2 antibody validation in neurodegenerative disease models"**
- **作者**: Doe J., et al.
- **摘要**: 该研究验证了一种新型GRIA2抗体的特异性，通过免疫印迹和免疫组化技术证明其在阿尔茨海默病模型小鼠脑组织中能特异性识别GRIA2蛋白，并发现疾病状态下GRIA2表达显著下调。
2. **"Localization of GRIA2 subunits in human brain tissue using a monoclonal antibody"**
- **作者**: Smith A., et al.
- **摘要**: 利用单克隆GRIA2抗体进行免疫荧光染色，揭示了GRIA2受体在人类前额叶皮层和海马体中的突触后分布，为研究谷氨酸能信号通路异常提供了工具。
3. **"GRIA2 autoantibodies in autoimmune encephalitis: clinical implications"**
- **作者**: Johnson R., et al.
- **摘要**: 研究报道了GRIA2自身抗体在自身免疫性脑炎患者血清中的存在，提示其可能与认知功能障碍相关，并开发了基于该抗体的诊断检测方法。
4. **"Characterization of a commercial GRIA2 antibody for synaptic protein analysis"**
- **作者**: Lee S., et al.
- **摘要**: 通过对比实验评估了市售GRIA2抗体的交叉反应性和灵敏度，发现其在啮齿类动物脑组织裂解液中表现优异，但在非还原条件下可能出现非特异性条带。
(注：以上为模拟内容，实际文献需通过PubMed等数据库查询。)
The GRIA2 antibody targets the GluA2 subunit of the α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptor, a critical ionotropic glutamate receptor in the central nervous system. Encoded by the GRIA2 gene, GluA2 plays a pivotal role in mediating fast synaptic transmission, synaptic plasticity, and learning/memory processes. A hallmark feature of GluA2 is its regulation of calcium permeability: most AMPA receptors containing GluA2 are impermeable to calcium due to RNA editing at the Q/R site, preventing excitotoxicity. Dysregulation of GRIA2 expression or function has been implicated in neurological disorders such as Alzheimer's disease, epilepsy, ischemic stroke, and neuropathic pain.
GRIA2 antibodies are widely used in neuroscience research to study receptor localization, trafficking, and post-translational modifications. They enable detection of GluA2 in techniques like Western blot, immunohistochemistry, and immunofluorescence, aiding investigations into synaptic changes under pathological conditions. Additionally, autoantibodies against GRIA2 have been identified in autoimmune encephalitis and paraneoplastic syndromes, where they disrupt synaptic signaling, leading to cognitive deficits or seizures. These clinical associations highlight the antibody's dual role as both a research tool and a biomarker. Commercially available GRIA2 antibodies are typically raised against specific epitopes, with validation emphasizing specificity for GluA2 over other AMPA receptor subunits (GluA1. GluA3. GluA4).
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