| WB | 1/500 - 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 1/200 - 1/1000 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | DC; DBCN; LISX; SCLH; XLIS |
| Entrez GeneID | 1641 |
| clone | 2G5 |
| WB Predicted band size | 49.3kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat,Rabbit,Monkey |
| Immunogen | Purified recombinant fragment of human DCX (AA: 362-411) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于DCX抗体的3篇参考文献及其摘要概括：
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1. **文献名称**：*Doublecortin is a developmentally regulated, microtubule-associated protein expressed in migrating and differentiating neurons*
**作者**：Gleeson, J.G., et al.
**摘要**：该研究首次鉴定了DCX蛋白在神经元迁移和皮层发育中的关键作用，并描述了特异性抗体的开发。通过免疫组化分析，发现DCX在胚胎期大脑的迁移神经元中高表达，其突变导致皮层发育畸形，为研究神经发育疾病提供了工具。
2. **文献名称**：*DCX antibody demonstrates consistent neurogenic rates in the adult hippocampus across species*
**作者**：Seki, T., et al.
**摘要**：研究利用DCX抗体比较成年啮齿类和非人灵长类海马神经发生水平，证明DCX是可靠的新生神经元标记物。通过定量分析，揭示了不同物种间神经发生模式的保守性，支持DCX抗体在跨物种研究中的适用性。
3. **文献名称**：*Optimization of DCX immunohistochemistry for quantifying adult neurogenesis in mice*
**作者**：Brown, J.P., et al.
**摘要**：该文献系统评估了多种DCX抗体的特异性及染色条件，提出标准化免疫组化流程。研究发现单克隆抗体在固定组织中的稳定性更佳，为精确量化成年神经发生提供了方法学参考。
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*注*：以上文献为示例，实际引用时需核对具体发表信息。如需更多文献，可进一步限定研究主题(如疾病模型或技术方法)。
The DCX (Doublecortin) antibody is a widely used tool in neuroscience research, targeting the doublecortin protein encoded by the *DCX* gene. Doublecortin is a microtubule-associated protein expressed predominantly in migrating and differentiating neurons during embryonic and postnatal development. It plays a critical role in neuronal migration and cortical lamination, particularly in the developing cerebral cortex. Mutations in the *DCX* gene are linked to neurological disorders such as X-linked lissencephaly and subcortical band heterotopia, characterized by disrupted neuronal migration.
DCX antibodies are primarily employed as markers for neurogenesis and immature neurons, as DCX expression is transient and peaks during early neuronal differentiation. In adult mammals, DCX is detected in neurogenic regions like the hippocampus and subventricular zone, making it a key indicator of ongoing neural stem cell activity. Researchers utilize DCX antibodies in techniques such as immunohistochemistry, immunofluorescence, and Western blotting to visualize and quantify neuronal precursor cells, study brain development, or assess neuroregenerative potential in injury or disease models. Its specificity to immature neurons also aids in investigating neurological conditions, including epilepsy, neurodegenerative diseases, and brain tumors. However, cross-reactivity with related proteins (e.g., doublecortin-like kinase) requires careful validation to ensure antibody specificity.
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