| WB | 1/500 - 1/2000 | Human,Monkey |
| IF | 咨询技术 | Human,Monkey |
| IHC | 咨询技术 | Human,Monkey |
| ICC | 技术咨询 | Human,Monkey |
| FCM | 1/200 - 1/400 | Human,Monkey |
| Elisa | 1/10000 | Human,Monkey |
| Aliases | PP2CA; PP2Calpha; PP2C-ALPHA |
| Entrez GeneID | 5494 |
| clone | 7F12H6 |
| WB Predicted band size | 42.4kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Monkey |
| Immunogen | Purified recombinant fragment of human PPM1A (AA: 202-382) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是3篇与PPM1A抗体相关的文献概览，包含关键研究内容：
1. **"PPM1A controls AMPK-related autophagy through TSC2 dephosphorylation"**
- 作者：Chen et al. (2018)
- 摘要：研究使用特异性PPM1A抗体验证其在HEK293T细胞中的表达，发现PPM1A通过去磷酸化TSC2调控AMPK介导的自噬通路，揭示了其在能量代谢中的新机制。
2. **"Antibody-based profiling of protein phosphatase expression in hepatocellular carcinoma"**
- 作者：Li & Wang (2020)
- 摘要：通过Western blot和免疫组化分析肝癌组织，发现PPM1A抗体检测显示该蛋白在肿瘤组织中显著低表达，提示其可能作为肝细胞癌的潜在生物标志物。
3. **"PPM1A negatively regulates antiviral responses by dephosphorylating TBK1"**
- 作者：Zhang et al. (2019)
- 摘要：利用siRNA敲低和PPM1A抗体免疫沉淀实验，证明PPM1A通过去磷酸化TBK1抑制I型干扰素产生，揭示其在天然免疫应答中的负反馈作用。
**备注**：上述文献信息为示例性内容，实际引用时需通过PubMed或Web of Science核对原文准确性。若需具体文章，建议以"PPM1A antibody"+"Western blot"/"immunoprecipitation"为关键词检索近年文献。
The PPM1A antibody is a crucial tool for studying the protein phosphatase magnesium-dependent 1A (PPM1A), a member of the PP2C family of serine/threonine phosphatases. PPM1A plays a pivotal role in regulating cellular stress responses, cell cycle progression, and apoptosis by dephosphoryulating key signaling molecules. It is implicated in modulating pathways such as TGF-β, p38 MAPK, and NF-κB, impacting processes like inflammation, cancer progression, and metabolic disorders. Dysregulation of PPM1A has been linked to diseases including hepatocellular carcinoma, neurodegenerative conditions, and diabetes.
The PPM1A antibody enables the detection and quantification of PPM1A expression in various experimental applications, including Western blotting, immunohistochemistry, immunoprecipitation, and immunofluorescence. Researchers rely on its specificity to investigate PPM1A’s subcellular localization, interaction partners, and expression patterns across tissues or under pathological conditions. High-quality PPM1A antibodies are typically validated using knockout controls or siRNA-mediated knockdown to confirm target specificity.
Available as monoclonal or polyclonal versions from diverse host species (e.g., rabbit, mouse), these antibodies are essential for elucidating PPM1A’s regulatory mechanisms in cellular homeostasis and disease, offering potential insights for therapeutic targeting. Proper validation ensures reliability in both basic research and translational studies.
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