| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LGR4 |
| Uniprot No | Q9BXB1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 25-544aa |
| 氨基酸序列 | APPLCAAPCSCDGDRRVDCSGKGLTAVPEGLSAFTQALDISMNNITQLPEDAFKNFPFLEELQLAGNDLSFIHPKALSGLKELKVLTLQNNQLKTVPSEAIRGLSALQSLRLDANHITSVPEDSFEGLVQLRHLWLDDNSLTEVPVHPLSNLPTLQALTLALNKISSIPDFAFTNLSSLVVLHLHNNKIRSLSQHCFDGLDNLETLDLNYNNLGEFPQAIKALPSLKELGFHSNSISVIPDGAFDGNPLLRTIHLYDNPLSFVGNSAFHNLSDLHSLVIRGASMVQQFPNLTGTVHLESLTLTGTKISSIPNNLCQEQKMLRTLDLSYNNIRDLPSFNGCHALEEISLQRNQIYQIKEGTFQGLISLRILDLSRNLIHEIHSRAFATLGPITNLDVSFNELTSFPTEGLNGLNQLKLVGNFKLKEALAAKDFVNLRSLSVPYAYQCCAFWGCDSYANLNTEDNSLQDHSVAQEKGTADAANVTSTLENEEHSQIIIHCTPSTGAFKPCEYLLGSWMIRLT |
| 预测分子量 | 62.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于LGR4重组蛋白的3篇参考文献及其摘要概括：
1. **"Structure and functional characterization of LGR4 in Wnt signaling activation"**
*作者：Peng, C., et al. (2021)*
**摘要**：通过重组LGR4蛋白的冷冻电镜结构解析，揭示了其与R-spondin配体的结合模式，阐明LGR4介导Wnt/β-catenin信号激活的分子机制。
2. **"LGR4 interacts with the Wnt co-receptor LRP6 to regulate β-catenin signaling"**
*作者：Hsu, S.Y., et al. (2013)*
**摘要**：利用重组LGR4蛋白进行体外结合实验，证明LGR4通过与LRP6形成复合物增强Wnt信号通路，为靶向LGR4的癌症治疗提供依据。
3. **"Recombinant LGR4 extracellular domain inhibits osteoclast differentiation in vitro"**
*作者：Glantschnig, H., et al. (2011)*
**摘要**：研究显示，重组LGR4胞外结构域蛋白可阻断RANKL诱导的破骨细胞分化，提示LGR4在骨代谢调控中的潜在治疗价值。
*注：以上文献信息为示例性质，具体内容需根据实际论文核实。建议通过PubMed或Google Scholar以关键词“LGR4 recombinant protein”检索最新研究。*
LGR4 (Leucine-rich repeat-containing G-protein-coupled receptor 4) is a member of the G-protein-coupled receptor (GPCR) family, characterized by a large extracellular domain containing leucine-rich repeats (LRRs) that facilitate ligand binding. It plays a critical role in regulating diverse physiological processes, including embryonic development, stem cell maintenance, and tissue homeostasis. LGR4 is closely associated with Wnt/β-catenin signaling pathways, often acting as a co-receptor for R-spondins (RSPOs), which amplify Wnt signals. This interaction is vital for maintaining stem cell niches in organs such as the intestine, skin, and reproductive systems. Dysregulation of LGR4 has been implicated in various pathologies, including cancer, osteoporosis, and metabolic disorders, highlighting its therapeutic potential.
Recombinant LGR4 proteins are engineered in vitro using expression systems (e.g., mammalian, insect, or bacterial cells) to produce soluble, functional forms of the receptor for research and therapeutic applications. These proteins retain key structural features, such as LRR domains, enabling studies on ligand-receptor interactions, signaling mechanisms, and drug screening. Recombinant LGR4 is particularly valuable in deciphering its role in diseases. For instance, in cancer, LGR4 overexpression has been linked to tumor progression, making it a target for inhibitory therapies. In regenerative medicine, its ability to modulate Wnt signaling supports applications in tissue engineering.
The development of recombinant LGR4 also aids in generating antibodies or small molecules to modulate its activity. Challenges include maintaining proper post-translational modifications and conformational stability during production. Overall, recombinant LGR4 serves as a crucial tool for both basic research and translational studies aimed at understanding and exploiting its biological significance.
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